Effects of fibronectin and laminin on structural, mechanical and transport properties of 3D collageneous network
Effects of fibronectin and laminin on structural, mechanical and transport properties of 3D collageneous network
复制标题
DOI：
10.1007/s10856-006-0686-5
复制
发表时间：
2007-02-01
影响因子：
3.7
通讯作者：
Netti, P. A.
中科院分区：
文献类型：
--
作者：
Guarnieri, D.;Battista, S.;Netti, P. A.
最近的研究表明，在3D胶原凝胶中培养的细胞，除了其众所周知的生物化学作用，纤连蛋白（FN）和层粘连蛋白（LM）通过与胶原分子相互作用改变基质的机械和结构特性来影响细胞功能。虽然FN和LM的生物化学性质已被广泛研究，但很少有人知道它们在胶原基质组装中的作用。这项工作的目的是表征基于FN和LM的胶原蛋白半互穿聚合物网络（半IPN），以了解这些生物大分子物种如何影响胶原蛋白网络组装和特性。胶原凝胶和FN和LM为基础的胶原半互穿网络的形态，粘弹性和扩散性进行了分析，共聚焦激光扫描显微镜（CLSM），环境扫描电子显微镜（ESEM），透射电子显微镜（TEM），流变学和荧光恢复后光漂白（FRAP）技术。结果发现，FN和LM组织在聚集体中，散布在胶原凝胶中，并在细纤维中，沿着胶原纤维分布。此外，高FN和LM浓度影响胶原纤维的组装和结构，并诱导对流变学和运输性能的剧烈影响。
Recent studies, on cells cultured in 3D collagen gels, have shown that, beside from their well known biochemical role, fibronectin (FN) and laminin (LM) affect cell functions via a modification of mechanical and structural properties of matrix due to interaction with collagen molecules. Though biochemical properties of FN and LM have been widely studied, little is known about their role in collagen matrix assembly. The aim of this work was to characterize FN- and LM-based collagen semi-interpenetrating polymer networks (semi-IPNs), in order to understand how these biomacromolecular species can affect collagen network assembly and properties. Morphology, viscoelasticity and diffusivity of collagen gels and FN- and LM-based collagen semi-IPNs were analysed by Confocal Laser Scanning microscopy (CLSM), Environmental Scanning Electron microscopy (ESEM), Transmission Electron microscopy (TEM), Rheometry and Fluorescence Recovery After Photobleaching (FRAP) techniques. It was found that FN and LM were organized in aggregates, interspersed in collagen gel, and in thin fibrils, distributed along collagen fibres. In addition, high FN and LM concentrations affected collagen fibre assembly and structure and induced drastic effects on rheological and transport properties.