Three-dimensional structure and flexibility of proteins of the RCA family - a progress report
Three-dimensional structure and flexibility of proteins of the RCA family - a progress report
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DOI：
10.1042/bst0300990
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发表时间：
2002-11-01
影响因子：
3.9
通讯作者：
Barlow, PN
中科院分区：
文献类型：
--
作者：
Herbert, A;O'Leary, J;Barlow, PN
补体激活调节蛋白(RCA)家族成员在健康和疾病中起着至关重要的作用。在这份报告中，我们描述了我们为解决人类膜辅助因子蛋白(CD46)、痘苗病毒补体控制蛋白(模仿哺乳动物RCA蛋白)和人类补体受体1型(CD35)的结构所做的努力。这些例子表明，尽管在过去的十年中取得了很好的进展，但补体作为细胞外多结构域糖蛋白的调节因子仍然给结构生物学家带来了巨大的问题。许多重要的问题仍然没有得到回答，特别是关于这些蛋白质的灵活性以及它们在接触结合伙伴时经历构象重排的程度。
Members of the regulator of complement activation (RCA) protein family perform a vital role in health and disease. In this report we describe our efforts to solve the structures of human membrane cofactor protein (CD46), the vaccinia virus complement control protein, which mimics mammalian RCA proteins, and human complement receptor type 1 (CD35). These examples illustrate that, despite good progress over the last decade, the regulators of complement, as extracellular multiple domain glycoproteins, still pose formidable problems to structural biologists. Many important questions remain unanswered, in particular with regard to the flexibility of these proteins and the extent to which they undergo conformational rearrangements on engaging their binding partners.