Integrated miRNAome and Transcriptome Analysis Reveals Argonaute 2-Mediated Defense Responses Against the Devastating Phytopathogen Sclerotinia sclerotiorum
Integrated miRNAome and Transcriptome Analysis Reveals Argonaute 2-Mediated Defense Responses Against the Devastating Phytopathogen Sclerotinia sclerotiorum
复制标题
整合的 miRNA 组和转录组分析揭示了 Argonaute 2 介导的针对毁灭性植物病原体核盘菌的防御反应
DOI：
10.3389/fpls.2020.00500
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发表时间：
2020-04-29
影响因子：
5.6
通讯作者：
Cai, Xin-Zhong
中科院分区：
文献类型：
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作者：
Cao, Jia-Yi;Xu, You-Ping;Cai, Xin-Zhong
ArgAerte 2(AGO2)介导的植物对真菌病原体的防御作用在很大程度上仍不清楚。本研究利用agO2突变体对miRNAome和转录组进行整合分析，以揭示agO2相关的miRNAs和对毁灭性的营养坏死性植物病原菌核盘菌的防御反应。对接种核盘菌的AGO2-1突变体(AGO2-SS)和野生型(WT-SS)以及模拟接种的AGO2-1突变体(AGO2)和野生型(WT)拟南芥的miRNAome和转录产物进行了序列分析。鉴定了WT-SS/WT、AGO2/WT、AGO2-SS/WT和AGO2-SS/AGO2的差异表达基因(DEG)和差异表达的miRNAs(DEM)。此外，对DEM和DEGS的整合分析确定了40多个潜在的依赖AGO2的菌核反应(ATSR)DEM-DEG对，涉及免疫识别、钙通量、氧化还原动态平衡、激素积累和信号转导、细胞壁修饰和金属离子动态平衡的调节。数据挖掘结果表明，大多数DEM与AGO2结合。此外，拟南芥突变体分析表明，已鉴定的DEM-DEG对GSTU2、GSTU5和RBOHF中与ROS和氧化还原稳态相关的三个基因参与了AGO2介导的对核盘菌的防御。这项工作提供了全基因组范围的miRNA目标基因对的预测，这些基因对可能与AGO2依赖的对菌核病的抗性有关。
Argonaute 2 (AGO2)-mediated role in plant defense against fungal pathogens remains largely unknown. In this study, integrated miRNAome and transcriptome analysis employing ago2 mutant was performed to reveal AGO2-associated miRNAs and defense responses against the devastating necrotrophic phytopathogen Sclerotinia sclerotiorum. Both miRNAome and transcriptomes of S. sclerotiorum-inoculated ago2-1 mutant (ago2-Ss) and wild-type (WT-Ss) as well as mock-inoculated ago2-1 mutant (ago2) and wild-type (WT) Arabidopsis plants, were analyzed by sRNA and mRNA deep sequencing. Differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) of the comparisons WT-Ss/WT, ago2/WT, ago2-Ss/WT-Ss, and ago2-Ss/ago2 were identified. Furthermore, integration analysis for the DEMs and DEGs identified over 40 potential AGO2-dependent Sclerotinia sclerotiorum-responsive (ATSR) DEM-DEG pairs involving modulation of immune recognition, calcium flux, redox homeostasis, hormone accumulation and signaling, cell wall modification and metal ion homeostasis. Data-mining result indicated that most of the DEMs were bound with AGO2. Moreover, Arabidopsis mutant analysis demonstrated that three ROS and redox homeostatasis related DEGs of identified DEM-DEG pairs, GSTU2, GSTU5, and RBOHF contributed to the AGO2-mediated defense against S. sclerotiorum. This work provides genome-wide prediction of miRNA–target gene pairs that are potentially associated with the AGO2-dependent resistance against S. sclerotiorum.