Tritiated thymidine autoradiographic study on cellular migration in the gastric gland of the golden hamster
Tritiated thymidine autoradiographic study on cellular migration in the gastric gland of the golden hamster
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氚胸苷放射自显影对金黄地鼠胃腺细胞迁移的研究
DOI：
10.1007/bf00226025
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发表时间：
1976
影响因子：
3.6
通讯作者：
Prof. S. Fujita
中科院分区：
文献类型：
--
作者：
Dr. T. Hattori;Prof. S. Fujita
10只仓鼠重复注射~ 3 H-胸腺嘧啶核苷4天，分别存活7、28、42和100天。本文观察了注射~ 3 H-胸腺嘧啶核苷后不同时间胃腺壁细胞和颈粘液细胞的空间分布和标记指数的变化，并对颈粘液细胞的命运、主细胞的替代和细胞迁移方式进行了讨论。从颈部开始，它们平均每天向下迁移3微米。到42天，它们到达腺体的中层，标记的粘液颈细胞减少，但标记的主细胞数量增加。然后，主细胞的分化似乎通过迁移性粘液颈细胞的转化在腺体的中层发生。标记4天后，发现位于腺体下部的约1.8%的主细胞进行原位复制。这表明这种细胞类型的更新部分是由其自身的有丝分裂活动保证的。小凹细胞--未来的表面上皮--似乎在管道系统中沿腺小管的长轴沿着向上迁移，这意味着“先产生，先迁移”。壁细胞和粘液颈细胞（未来的主细胞）从颈部迁移出来后，平均需要200天才能到达腺体的下端。然而，在迁移过程中，在颈部同时产生的细胞从颈部向腺体底部分散分布。新形成的细胞到达腺体下端所需的时间在100至300天之间。在胃腺中，首先在颈部产生的细胞并不首先到达腺体的下端。这种随机迁移模式被称为“随机流动系统”。垂直于腺小管长轴的细胞运动是影响细胞向下迁移的可能因素之一，它在胃腺的任何水平上都是随机发生的。
Ten hamsters received repeated injections of3H-thymidine for 4 days and were allowed to survive for 7, 28, 42 and 100 days. Changes in spatial distribution of the labelled cells and in labelling indices of each cell line in the gastric glands were studied at various days after3H-thymidine injections, and the fate of the mucous neck cell, the replacement of the chief cell and the mode of cell migration were discussed.After 4 days of repeated injections of3H-thymidine, the labelled parietal cells and the mucous neck cells were concentrated at the neck area. Starting from the neck area, they migrated an average of 3 micra downwards per day. By 42 days, they reached the middle level of the glands, where the labelled mucous neck cells decreased but the labelled chief cells increased in number. The differentiation of the chief cell then appears to take place at the middle level of the glands through transformation of the migratory mucous neck cells. After 4 days of the labelling, about 1.8% of the chief cells located in the lower part of the glands was found to undergo in situ replication. This indicates that the renewal of this cell type is partly assured by its own mitotic activity.The foveolar cell — the future surface epithelium — seems to migrate upwards along the long axis of the glandular tubule in the pipe line system, which means “first produced, first migrates”. After migrating out from the neck area, the parietal cell and the mucous neck cell (the future chief cell) take an average of 200 days to reach the lower end of the glands. In the process of migration, however, the cells produced contemporaneously at the neck area became scatteringly spread from the neck towards the bottom of the gland. The time required for the newly-formed cells to reach the lower end of the gland varied between 100 and 300 days. In the gastric glands the cells first produced at the neck area do not first reach the lower end of the glands. This mode of random migration is referred to as the “stochastic flow system”. As one of the probable factors which disturb the pipe line flow of downward cell migration, cellular movements perpendicular to the long axis of the glandular tubule were suggested to occur at random at an any level of the gastric glands.