Genome-wide identification of heat shock proteins (Hsps) and Hsp interactors in rice: Hsp70s as a case study.
Genome-wide identification of heat shock proteins (Hsps) and Hsp interactors in rice: Hsp70s as a case study.
复制标题
水稻中热休克蛋白 (Hsps) 和 Hsp 相互作用因子的全基因组鉴定：以 Hsp70s 为例
DOI：
10.1186/1471-2164-15-344
复制
发表时间：
2014-05-07
期刊：
影响因子：
4.4
通讯作者：
He H
中科院分区：
文献类型：
--
作者：
Wang Y;Lin S;Song Q;Li K;Tao H;Huang J;Chen X;Que S;He H
热激蛋白（Heat shock proteins，Hsps）在保护植物免受非生物胁迫中起着重要作用。尽管研究人员在Hsps家族成员的功能分析方面做了大量的工作，但在水稻中Hsps的功能还没有得到充分的研究。结果在本研究中，我们将基于同源性的方法与表达关联数据相结合，筛选出与热响应探针集表达模式密切相关的水稻Hsps。27个Hsp候选者被鉴定，包括12个小Hsps，6个Hsp 70，3个Hsp 60，3个Hsp 90和3个clpB/Hsp 100。然后，结合interlog和expression profile的方法，推断了水稻Hsp 70的430个互作因子，并通过共定位和功能分析验证了互作效应。随后的分析表明，13个相互作用的结构域和28个目标模体在热休克蛋白70的相互作用过度。24个生物学过程的GO术语和5个分子功能的GO术语在正相互作用物中富集，其表达水平与Hsp 70 s正相关。Hsp 70 s相互作用网络揭示了Hsp 70 s参与大分子转运、碳水化合物代谢、先天免疫、光系统II修复和激酶活性调节等功能。结论鉴定了27种水稻Hsp 70 s，推测并验证了430种Hsp 70 s相互作用因子，构建了Hsp 70 s相互作用网络，分析了Hsp 70 s的功能。此外，还建立了两个数据库，名为水稻热激蛋白（RiceHsps）和水稻基因表达谱（RGEP），以及一个在线工具，名为蛋白质-蛋白质相互作用预测（PPIP），可以在 http://bioinformatics.fafu.edu.cn/ .
BackgroundHeat shock proteins (Hsps) perform a fundamental role in protecting plants against abiotic stresses. Although researchers have made great efforts on the functional analysis of individual family members, Hsps have not been fully characterized in rice (Oryza sativaL.) and little is known about their interactors.ResultsIn this study, we combined orthology-based approach with expression association data to screen rice Hsps for the expression patterns of which strongly correlated with that of heat responsive probe-sets. Twenty-seven Hsp candidates were identified, including 12 small Hsps, six Hsp70s, three Hsp60s, three Hsp90s, and three clpB/Hsp100s. Then, using a combination of interolog and expression profile-based methods, we inferred 430 interactors of Hsp70s in rice, and validated the interactions by co-localization and function-based methods. Subsequent analysis showed 13 interacting domains and 28 target motifs were over-represented in Hsp70s interactors. Twenty-four GO terms of biological processes and five GO terms of molecular functions were enriched in the positive interactors, whose expression levels were positively associated with Hsp70s. Hsp70s interaction network implied that Hsp70s were involved in macromolecular translocation, carbohydrate metabolism, innate immunity, photosystem II repair and regulation of kinase activities.ConclusionsTwenty-seven Hsps in rice were identified and 430 interactors of Hsp70s were inferred and validated, then the interacting network of Hsp70s was induced and the function of Hsp70s was analyzed. Furthermore, two databases named Rice Heat Shock Proteins (RiceHsps) and Rice Gene Expression Profile (RGEP), and one online tool named Protein-Protein Interaction Predictor (PPIP), were constructed and could be accessed at http://bioinformatics.fafu.edu.cn/ .
登录
查看更多内容
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
--
作者：
Fisher, RA
通讯作者：
Fisher, RA
agriGO：农业界的 GO 分析工具包
影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4.8
作者：
Gao, TY;Newton, AC
通讯作者：
Newton, AC