Use of molecular markers in reciprocal recurrent selection of maize increases heterosis effects
Use of molecular markers in reciprocal recurrent selection of maize increases heterosis effects
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DOI：
10.4238/2011.october.25.6
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发表时间：
2011-01-01
影响因子：
0.4
通讯作者：
do Amaral Junior, A. T.
中科院分区：
文献类型：
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作者：
Berilli, A. P. C. G.;Pereira, M. G.;do Amaral Junior, A. T.
为了最大限度地提高玉米交互轮回选择的杂种优势和寿命，我们研究了分子标记对群体间和群体内变异的影响。分子变异性进行了量化间简单序列重复（ISSR）标记之间和玉米群体Cimmyt和Piranao在第10个周期的互惠轮回选择程序。每个群体的42个S-1后代进行了分析，这些是根据其农艺性状选择的全同胞家系。共筛选出13个引物，共扩增出140条带，其中多态性条带114条，单态条带26条。根据UPGMA聚类分析和遗传距离，可以对“污染”后代进行鉴定。这些后代属于Piranao或Cimmyt类群，但聚在相反的杂种优势群中。“污染”后代的鉴定与选择相关，因为除了鉴定在重组阶段应该消除的基因型之外，它还允许在遗传上不同的个体之间的杂交中增加杂种优势表达。在排除“污染”后代和那些分配在杂种优势群之间的后代后，进行了新的统计分析，表明群体之间的遗传距离增加。得出的结论是，在互惠轮回选择程序中的分子标记的应用，允许优化监测群体内和群体之间的遗传变异，有利于更远的后代之间的重组，除了确保增加寿命的互惠轮回选择程序。
We examined the effect of incorporation of molecular markers on variability between and within populations in order to maximize heterotic effects and longevity of a maize reciprocal recurrent selection program. Molecular variability was quantified by inter-simple sequence repeat (ISSR) markers between and within the maize populations Cimmyt and Piranao in the 10th cycle of a reciprocal recurrent selection program. Forty-two S-1 progenies of each population were analyzed, these being families of full-sibs selected according to their agronomic traits. Thirteen primers were selected, which produced 140 bands; 114 of them were polymorphic and 26 monomorphic. Based on UPGMA grouping analysis and by genetic distances, it was possible to identify "contaminant" progenies. These progenies belong to the Piranao or Cimmyt groups, but cluster in the opposite heterotic group. Identification of "contaminant" progenies is relevant for selection, because, besides identifying genotypes that should be eliminated at the recombination stage, it allows increased heterosis expression in crosses between more genetically distinct individuals. After the elimination of the "contaminant" progenies and those that were allocated between the heterotic groups, a new statistical analysis was carried out, which demonstrated increased genetic distances between the populations. It was concluded that the application of molecular markers in reciprocal recurrent selection programs allows the optimization of the monitoring of genetic variability within and between populations, favoring recombination between more distant progenies, besides ensuring increased longevity of the reciprocal recurrent selection program.