Phylogeography of Rhabdias spp. (Nematoda: Rhabdiasidae) collected from Bufo species in Honshu, Shikoku, and Kyushu, Japan including possible cryptic species
Phylogeography of Rhabdias spp. (Nematoda: Rhabdiasidae) collected from Bufo species in Honshu, Shikoku, and Kyushu, Japan including possible cryptic species
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横纹藻属的系统发育地理学。
DOI：
10.1016/j.parint.2022.102612
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发表时间：
2022
影响因子：
1.9
通讯作者：
Urabe Misako
中科院分区：
文献类型：
--
作者：
Marcaida Arvin Jet B.;Nakao Minoru;Fukutani Kazumi;Nishikawa Kanto;Urabe Misako
RhabdiasSiles&Hassall属，1905年，由世界各地两栖和爬行动物的肺部寄生虫组成。在日本已记录到9种，其中包括1930年仅在东亚钳蝎属中报道的Rhabdias incertaWilkie。在本研究中，评估OFR的多样性。在此基础上，我们对采自日本蟾蜍三个种/亚种的横纹盘虫进行了分子分析，分别为中华大蟾蜍、台湾大蟾蜍和台湾大蟾蜍。Torrenticola，采集于日本本州、四国岛和九州的不同地区。用线粒体DNA(COI)和核DNA(28S)比较DNA序列差异，以确定可能的隐蔽物种。通过光学显微镜和扫描电子显微镜(SEM)进行形态分析。结果表明，Bufospp.作为至少三种轮虫的寄主。在形态上，大多数标本被鉴定为ASR。Incertaas incertaas似乎是东亚钳鼠属特有的物种，并根据其寄主的亚种划分和生物地理被进一步细分为两个或三个系统群。在本研究中收集的一些Rhabdias标本与R.TokyoenssWilkie，1930，寄生在尾目的寄主中，这表明寄主切换。分子和形态分析都表明，在日本采集的蟾蜍中存在未描述的和隐蔽的横纹虫物种。这项研究是日本第一次对横纹夜蛾进行分子特征描述，包括新的OFR序列。确定的和两个未描述的物种。
The genusRhabdiasStiles & Hassall, 1905 comprises lung parasites of amphibians and reptiles worldwide. In Japan, 9 species have been recorded, includingRhabdias incertaWilkie, 1930 which has been reported only inBufospecies. In this study, to assess the diversity ofR. incerta, we performed molecular analyses ofRhabdiasspecies sampled from three species/subspecies of Japanese toads namelyBufo japonicus,B. japonicus formosus, andB. torrenticola, collected in various regions of Honshu, Shikoku, and Kyushu, Japan. DNA sequence divergence was compared using mtDNA (COI) and nuclear DNA (28S) to identify possible cryptic species. Morphological analysis was performed through light microscopy and scanning electron microscopy (SEM). The results revealed thatBufospp. serve as hosts for at least threeRhabdiasspecies. Morphologically, most samples were identified asR. incertabut with a longer body and larger buccal cavity than originally described.Rhabdias incertaappears to be specific to the genusBufoand is further subdivided into two or three phylogroups based on subspecies divisions and biogeography of their host. SomeRhabdiasspecimens collected in this study resembleR. tokyoensisWilkie, 1930, parasitic in hosts from the order Caudata, which suggests host switching. Both molecular and morphological analyses suggested the presence of undescribed and crypticRhabdiasspecies within toads collected in Japan. This study was the first to molecularly characterizeRhabdiasspecies in Japan, including novel sequences ofR. incertaand two undescribed species.