Expression of an Activated Form of Integrin beta2 Chain CD18 in Cardiac Surgical Operations.
Expression of an Activated Form of Integrin beta2 Chain CD18 in Cardiac Surgical Operations.
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DOI：
10.14712/18059694.2017.81
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发表时间：
2007-01-01
影响因子：
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通讯作者：
Krejsek, Jan
中科院分区：
文献类型：
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作者：
Kudlova, Manuela;Kolackova, Martina;Krejsek, Jan
背景：髓样细胞在接受心脏外科手术的患者中被广泛激活。据推测，这种激活在心肺转流（CPB）手术患者中更为深刻。（“体外循环”）与非CPB手术患者相比目的：评价在由MEM-148抗体识别的髓样细胞上表达的新活化标志物的表达变化。采用流式细胞术检测40例冠状动脉旁路移植术（CABG）患者的MEM-148阳性髓系细胞的表达，并随机分为“体外循环”和“非体外循环”两组。在“体外循环”手术期间，MEM-148阳性骨髓细胞的相对和绝对数量显着减少。他们的数量显着增加，在术后期间，在“上泵”和“关闭泵”patients. There之间没有显着差异“上泵”和“关闭泵”patients.CONCLUSIONS：非常创伤的手术似乎是更相关的髓系细胞的激活，他们CPB本身开始。
BACKGROUND: Myeloid cells are extensively activated in patients undergoing cardiosurgical operations. It is supposed that this activation is more profound in patients operated with cardiopulmonary bypass (CPB) ("on-pump") in comparison with patients operated without CPB ("off-pump").AIMS: To evaluate changes in the expression of a novel activation marker expressed on myeloid cells recognized by MEM-148 antibody.PATIENTS AND METHODS: The expression of MEM-148 positive myeloid cells was evaluated by flow cytometry in 40 patients who underwent coronary artery bypass surgery (CABG) randomly assigned to "on-pump" or "off-pump" technique.RESULTS: The relative and absolute number of MEM-148 positive myeloid cells is significantly diminished during "on-pump" surgery. A significant increase in their number in postoperative period in both "on-pump" and "off-pump" patients was found. There were no significant differencies between "on-pump" and "off-pump" patients.CONCLUSIONS: The very trauma of surgery seems to be more relevant in starting on activation of myeloid cells them CPB itself.