AUTOMATED ACTIN FILAMENT SEGMENTATION, TRACKING AND TIP ELONGATION MEASUREMENTS BASED ON OPEN ACTIVE CONTOUR MODELS.
AUTOMATED ACTIN FILAMENT SEGMENTATION, TRACKING AND TIP ELONGATION MEASUREMENTS BASED ON OPEN ACTIVE CONTOUR MODELS.
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DOI：
10.1109/isbi.2009.5193303
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发表时间：
2009-06-28
期刊：
影响因子：
--
通讯作者：
Huang X
中科院分区：
文献类型：
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作者：
Li H;Shen T;Smith MB;Fujiwara I;Vavylonis D;Huang X
本文提出了一种自动化的方法，肌动蛋白丝分割和跟踪测量尖端伸长率在全内反射荧光显微镜（TIRFM）的图像。本文的主要贡献是：（1）采用一种新的开放式活动轮廓模型进行细丝分割和跟踪，该模型具有快速性和抗噪声能力强的特点：（2）提出了不同的策略来解决细丝相交问题，这是细丝跟踪的主要困难;以及（iii）这种完全自动化的方法避免了人工交互的需要，从而减少了图像序列上的整个伸长测量过程所需的时间。实验结果表明了该方法的鲁棒性和有效性。
This paper presents an automated method for actin filament segmentation and tracking for measuring tip elongation rates in Total Internal Reflection Fluorescence Microscopy (TIRFM) images. The main contributions of the paper are: (i) we use a novel open active contour model for filament segmentation and tracking, which is fast and robust against noise; (ii) different strategies are proposed to solve the filament intersection problem, which is shown to be the main difficulty in filament tracking; and (iii) this fully automated method avoids the need of human interaction and thus reduces required time for the entire elongation measurement process on an image sequence. Application to experimental results demonstrated the robustness and effectiveness of this method.