Phylogenetic diversity and structure of sebacinoid fungi associated with plant communities along an altitudinal gradient
Phylogenetic diversity and structure of sebacinoid fungi associated with plant communities along an altitudinal gradient
复制标题
DOI：
10.1111/j.1574-6941.2012.01473.x
复制
发表时间：
2013-02-01
影响因子：
4.2
通讯作者：
Weiss, Michael
中科院分区：
文献类型：
--
作者：
Garnica, Sigisfredo;Riess, Kai;Weiss, Michael
为了研究德国巴伐利亚阿尔卑斯山脉8个植被群落的Sebacinales群落的多样性和系统发育结构，我们分析了456个植物的菌体或根。我们检测到264个类皮脂素序列，跨越核rRNA基因的基因间转录间隔区，5.8S和D1/D2区域，主要采用巢式PCR方法。在97%序列相似度的基础上，从44科70种寄主中获得73个Sebacinales分子分类单位。26个分子分类单位代表单种，其中最常见的是只局限于森林或草地生境。虽然在植物根系中出现的Sebacinales丰度很低，但这些微生物在系统发育上是多样的，并且在所研究的生态系统中广泛分布。排序分析表明，土地利用方式、pH值和腐殖质含量对油脂碱属植物的多样性和聚集有显著影响。在极端土壤条件或集约利用的生态系统中，Sebacinales群落表现出明显的系统发育聚类，而在未受干扰的植物群落中则没有这种趋势。这些结果表明，生态系统干扰和环境力量在局部和空间尺度上都影响着黄芪群落群落的多样性和结构。
To study the diversity and phylogenetic structure of Sebacinales communities from eight vegetation communities along an altitudinal gradient in the Bavarian Alps (Germany), we analysed 456 thalli or roots of plants. We detected 264 sebacinoid sequences, spanning the intergenic transcribed spacer region, 5.8S and D1/D2 regions of the nuclear rRNA gene, mostly using a nested PCR approach. Based on 97% sequence similarity, 73 Sebacinales molecular taxonomic units were found from 70 host species belonging to 44 plant families. Twenty-six molecular taxonomic units represented singletons, the most frequent of these being restricted exclusively to either wooded or grassland habitats. Although Sebacinales appear to occur in low abundance in plant roots, these microorganisms are phylogenetically diverse and widely spread in the ecosystems studied. Ordination analyses showed that land use, pH and humus content strongly influence the diversity and assembly of Sebacinales. In most cases, Sebacinales communities in ecosystems with extreme soil conditions or intensive land use exhibited significant phylogenetic clustering, whereas in undisturbed plant communities no trend was observed. These results suggest that ecosystem disturbance and environmental forces have an influence on the diversity and structure of Sebacinales community assembly over local and spatial scales.