Mapping the landscape of transcription factor promoter activity during vegetative development in Marchantia
Mapping the landscape of transcription factor promoter activity during vegetative development in Marchantia
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DOI：
10.1101/2023.06.17.545419
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发表时间：
2023-06
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通讯作者：
Facundo Romani;Susanna Sauret-Güeto;M. Rebmann;D. Annese;Ignacy Bonter;Marta Tomaselli;Tom Dierschke;Mihails Delmans;Eftychios Frangedakis;Linda Silvestri;Jenna Rever;J. Bowman;Ignacio Romani;J. Haseloff
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作者：
Facundo Romani;Susanna Sauret-Güeto;M. Rebmann;D. Annese;Ignacy Bonter;Marta Tomaselli;Tom Dierschke;Mihails Delmans;Eftychios Frangedakis;Linda Silvestri;Jenna Rever;J. Bowman;Ignacio Romani;J. Haseloff
转录因子（TF）对于基因表达的调节和细胞命运的决定是必不可少的。表征TF基因在空间和时间上的转录活性是理解复杂生物系统的关键一步。芽苞植物的营养配子体分生组织与有花植物的茎尖分生组织具有某些共同的特征。然而，与配子体组织相关的TF的身份和表达谱在很大程度上是未知的。多形地钱（Marchantia polymorpha）是植物系统生物学的一个杰出的模式系统，其TF基因只有10450个。我们已经产生了一个近乎完整的收集来自地钱TF基因的启动子元件。我们实验测试了在植物报告融合的所有TF启动子的集合和系统地分析了在地钱芽的表达模式。这使我们能够建立一个精确的表达结构域的地图，并确定一组独特的TF在干细胞区表达，提供了新的见解的配子体分生组织的动态调节及其演变。此外，我们提供了一个在线数据库的表达模式的所有启动子的集合。我们预计，启动子元件的特点，在这里将是有用的细胞类型特异性表达，合成生物学应用，和功能基因组学。
Transcription factors (TFs) are essential for the regulation of gene expression and cell fate determination. Characterising the transcriptional activity of TF genes in space and time is a critical step towards understanding complex biological systems. The vegetative gametophyte meristems of bryophytes share some characteristics with the shoot-apical meristems of flowering plants. However, the identity and expression profiles of TFs associated with gametophyte organization are largely unknown. With only ∼450 TF genes, Marchantia polymorpha is an outstanding model system for plant systems biology. We have generated a near-complete collection of promoter elements derived from Marchantia TF genes. We experimentally tested in planta reporter fusions for all the TF promoters in the collection and systematically analysed expression patterns in Marchantia gemmae. This allowed us to build a map of precise expression domains and identify a unique set of TFs expressed in the stem-cell zone, providing new insight into the dynamic regulation of the gametophytic meristem and its evolution. In addition, we provide an online database of expression patterns for all promoters in the collection. We expect that the promoter elements characterised here will be useful for cell-type specific expression, synthetic biology applications, and functional genomics.