UV-vis and FTIR-ATR spectroscopic techniques to study the inclusion complexes of genistein with β-cyclodextrins
UV-vis and FTIR-ATR spectroscopic techniques to study the inclusion complexes of genistein with β-cyclodextrins
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DOI：
10.1016/j.jpba.2007.01.054
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发表时间：
2007-05-09
影响因子：
3.4
通讯作者：
Venuti, V.
中科院分区：
文献类型：
--
作者：
Crupi, V.;Ficarra, R.;Venuti, V.
研究了β-环糊精（beta-CyD）、（2-羟丙基）-β-环糊精（HP-beta-CyD）和甲基-β-环糊精（Me-beta-CyD）对染料木素（Gen）紫外吸收的影响。根据Higuchi和Connors报道的方法进行了相溶解度研究，以评估络合状态下的变化，所获得的图表明它形成化学计量比为1：1的络合物。采用共沉淀法制备了染料木素与这些大环化合物的1：1固体配合物，并通过红外吸收光谱进行了表征。在FTIR-ATR光谱中，观察到客体分子的某些谱带相对于1：1物理混合物和配合物的谱带发生了变化，特别是对高频O-H伸缩振动谱带进行了定量振动归属，从而证实了主客体间的相互作用。根据实验结果，对夹杂现象进行了讨论。(c)2007 Elsevier B. V.保留所有权利。
The effect of P-cyclodextrin (beta-CyD), (2-hydroxypropyl)-beta-cyclodextrin (HP-beta-CyD) and methyl-beta-cyclodextrin (Me-beta-CyD) complexation on the UV absorption of genistein (Gen) was studied in pure water. A phase solubility study was performed, according to the method reported by Higuchi and Connors, to evaluate the changes of isoflavone in the complexation state and the obtained diagrams suggested that it forms complexes with a stoichiometry of 1:1. Then, the solid complexes of genistein with these macrocycles in 1:1 molar ratio were prepared by the co-precipitation method and characterized by FTIR absorption spectroscopy in ATR geometry. The host-guest interactions have been evidenced by monitoring, in the FTIR-ATR spectra, the changes in some guest molecule bands relative to those observed in the spectra of the 1:1 physical mixtures and complexes.In particular, for the high-frequency O-H stretching band, a quantitative vibrational assignment of the observed sub-bands has been made. From the results, the inclusion phenomena have been discussed. (c) 2007 Elsevier B.V. All rights reserved.