Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway.
Girdin regulates the migration and invasion of glioma cells via the PI3K-Akt signaling pathway.
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吉尔丁通过PI3K-AKT信号通路调节神经胶质瘤细胞的迁移和侵袭。
DOI：
10.3892/mmr.2015.4049
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发表时间：
2015-10
影响因子：
3.4
通讯作者：
Tong X
中科院分区：
文献类型：
--
作者：
Ni W;Fang Y;Tong L;Tong Z;Yi F;Qiu J;Wang R;Tong X
Girdin是一种肌动蛋白结合蛋白，与细胞迁移有关，在胶质瘤细胞中高水平表达。然而，girdin与胶质瘤发生之间的关系仍有待阐明。本研究利用短发夹RNA技术沉默了girdin基因的表达。采用细胞活力测定、创面愈合试验、transwell侵袭试验、逆转录-定量聚合酶链反应、western blot分析和明胶酶谱法评估girdin沉默对胶质瘤细胞增殖、迁移和侵袭的影响。结果表明，沉默girdin可抑制胶质瘤细胞的增殖、迁移和侵袭。此外，基质金属蛋白酶(MMP)-2和MMP-9的表达水平和活性也受girdin沉默的影响。进一步的机制研究表明，girdin可能通过磷脂酰肌醇-3激酶/蛋白激酶B （PI3K-Akt）信号通路调节胶质瘤细胞的迁移和侵袭。因此，本研究结果为抗癌药物的开发提供了理论基础。
Girdin, an actin-binding protein, is associated with cell migration and is expressed at high levels in glioma cells. However, the association between girdin and the development of glioma remains to be elucidated. In the present study, short-hairpin RNA technology was used to silence the gene expression of girdin. The effects of girdin silencing on glioma cell proliferation, migration and invasion were then assessed using a cell viability assay, wound-healing assay, transwell invasion assay, reverse transcription-quantitative polymerase chain reaction, western blot analysis and gelatin zymography. The results suggested that girdin silencing inhibited the proliferation, migration and invasion of glioma cells. In addition, the expression levels and activity of matrix metalloproteinase (MMP)-2 and MMP-9 were also affected by girdin silencing. Further mechanistic investigation indicated that girdin may regulate glioma cell migration and invasion through the phosphatidylinositol-3-kinase/protein kinase B (PI3K-Akt) signaling pathway. Therefore, the results of the present study provide a theoretical foundation for the development of anticancer drugs.