Human neutrophil antigen-3a antibodies induce neutrophil aggregation in a plasma-free medium.
Human neutrophil antigen-3a antibodies induce neutrophil aggregation in a plasma-free medium.
复制标题
DOI：
10.2450/2013.0294-12
复制
发表时间：
2013-07
期刊：
影响因子：
--
通讯作者：
Nicole Schubert;Tom Berthold;Stefan Muschter;J. Wesche;B. Fürll;A. Reil;J. Bux;T. Bakchoul;A. Greinacher
中科院分区：
文献类型：
--
作者：
Nicole Schubert;Tom Berthold;Stefan Muschter;J. Wesche;B. Fürll;A. Reil;J. Bux;T. Bakchoul;A. Greinacher
背景抗人中性粒细胞同种异体抗原3a（HNA-3a）抗体参与了严重的输血相关急性肺损伤（TRALI）病例，但患者对HNA-3a抗体的敏感性差异很大。HNA-3a抗体诱导粒细胞聚集。然而，粒细胞聚集是否需要血浆蛋白质尚未解决。材料与方法我们通过使用和修改粒细胞凝集试验（GAT）来研究HNA-3a抗体诱导的多形核细胞聚集是否依赖于血浆因子。结果HNA-3a纯合子的多态性细胞在无血浆蛋白的GAT装置中与HNA-3a抗体孵育时不聚集。当使用重悬于含有蛋白质的磷酸盐缓冲盐水中的多形细胞进行GAT时，观察到HNA-3介导的聚集。此外，使用Tween® 20封闭板，在无蛋白质培养基中重建粒细胞聚集体。这表明粒细胞聚集可能通过粒细胞-粒细胞直接相互作用发生或由中性粒细胞活化后释放的物质介导。HNA-3a抗体诱导的粒细胞聚集不需要人血浆蛋白。HNA-3a抗体应答的个体间差异不依赖于患者血浆蛋白的差异。
BACKGROUND Antibodies against the human neutrophil alloantigen-3a (HNA-3a) are involved in severe cases of transfusion-related acute lung injury (TRALI), but the susceptibility of patients towards HNA-3a antibody differs largely. HNA-3a antibodies induce granulocyte aggregation. However, it is unresolved whether plasma proteins are required for granulocyte aggregation. MATERIALS AND METHODS We investigated whether HNA-3a-antibody-induced aggregation of polymorphonuclear cells is dependent on plasma factors by using and modifying the granulocyte agglutination test (GAT). RESULTS Polymorphonuclear cells homozygous for HNA-3a did not aggregate when incubated with HNA-3a antibodies in a plasma-protein-free GAT setup. When the GAT was performed using polymorphonuclear cells re-suspended in phosphate-buffered saline containing proteins, HNA-3-mediated aggregation was observed. Moreover, using Tween® 20 for blocking the plates, reconstituted the granulocyte aggregation in a protein-free medium. This indicates that granulocyte aggregation probably occurs by direct granulocyte-granulocyte interaction(s) or is mediated by substances released by neutrophils after activation. DISCUSSION Granulocyte aggregation induced by HNA-3a antibodies does not require human plasma proteins. Interindividual variability in the response to HNA-3a antibodies does not depend on differences in patient's plasma proteins.