Isolation and identification of bacteria and fungi growing spontaneously on polyhydroxyalkanoate pellets recovered by a new biological process
Isolation and identification of bacteria and fungi growing spontaneously on polyhydroxyalkanoate pellets recovered by a new biological process
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通过新的生物工艺回收的聚羟基脂肪酸酯颗粒上自发生长的细菌和真菌的分离和鉴定
DOI：
10.21161/mjm.81615
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发表时间：
2016
影响因子：
0.3
通讯作者：
H. Nagao
中科院分区：
文献类型：
--
作者：
S. Pyary;K. Sudesh;H. Nagao
目的：进行了研究以分离和鉴定生物回收的聚羟基链烷酸酯（PHA）共聚物聚（3-羟基丁酸酯-co-3羟基己酸酯）[P（3 HB-co-3 HHx）]表面上自发生长的细菌和真菌种群。方法和结果：使用300 L发酵罐进行大规模PHA生物合成，并利用我们实验室开发的生物方法回收PHA。使用标准微生物学和分子生物学技术，鉴定了生物回收PHA表面上自然生长的微生物种群。扫描电子显微镜（SEM）分析表明，鉴定的细菌（蜡状芽孢杆菌和洋葱伯克霍尔德氏菌）和真菌分离物（尼日尔曲霉、雪腐衣霉、桔青霉和灰黄青霉）能够在P（3 HB-co-3 HHx）共聚物上生长并降解P（3 HB-co-3 HHx）共聚物。研究结论、意义和影响：这是关于生物回收PHA颗粒的首次报告，涉及在实验室条件下储存期间微生物在其上自发生长的情况。真菌似乎在其定殖生物回收的PHA的能力方面优于细菌。
Aims: The study was carried out to isolate and identify the spontaneously growing populations of bacteria and fungi on the surface of biologically recovered polyhydroxyalkanoate (PHA) copolymer, poly(3-hydroxybutyrate-co-3hydroxyhexanoate) [P(3HB-co-3HHx)]. Methodology and results: Large-scale PHA biosynthesis was carried out using 300 L fermenter and a biological methodology developed in our laboratory was utilized for PHA recovery. Using standard microbiological and molecular biology techniques the naturally growing microbial populations on the surface of biologically recovered PHA were identified. Scanning electron microscopy (SEM) analysis showed that the identified bacterial (Bacillus cereus and Burkholderia cepacia) and fungal isolates (Aspergillus niger, Byssochlamys nivea, Penicillium citrinum and Penicillium griseofulvum) were able to grow on and degrade the P(3HB-co-3HHx) copolymer. Conclusion, significance and impact of study: This is the first report on biologically recovered PHA pellet addressing the occurrence of microorganisms that grew spontaneously on it during storage under laboratory conditions. Fungi appeared to be dominant over bacteria in their ability to colonize the biologically recovered PHA.
影响因子：
4.4
作者：
Frank, Jeremy A.;Reich, Claudia I.;Olsen, Gary J.
通讯作者：
Olsen, Gary J.