Repairing Transected Peripheral Nerve Using a Biomimetic Nerve Guidance Conduit Containing Intraluminal Sponge Fillers
Repairing Transected Peripheral Nerve Using a Biomimetic Nerve Guidance Conduit Containing Intraluminal Sponge Fillers
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DOI：
10.1002/adhm.201900913
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发表时间：
2019-10-04
影响因子：
10
通讯作者：
Li, Yi
中科院分区：
文献类型：
--
作者：
Hou, Yuanjing;Wang, Xinyu;Li, Yi
几何设计的神经导管（NGC）在引导周围神经损伤后的神经重建中显示出显著的优势。研究发现，具有独特结构的管腔内填充物可以有效地为轴突的出芽提供内在导向，并提高NGC的通透性。在这项工作中，制备了含有腔内海绵填充物的聚（乳酸-乙醇酸）（PLGA）NGC（标记为ISF-NGC），并用于重建具有10 mm间隙的大鼠坐骨神经。为了比较，将相同的程序应用于单个中空PLGA NGC（标记为H-NGC）和自体神经。如通过显著改善的神经形态和功能所证明的，ISF-NGC实现了优于H-NGC的上级神经修复效果，这与自体神经移植相当。H-NGC可能仅为神经纤维再生长和轴突延伸提供受保护的隧道，而ISF-NGC提供细胞外基质模拟结构作为自体移植物，为神经再支配提供接触引导。这种新开发的ISF-NGC是一种很有前途的候选者，可以帮助神经再支配穿过临床病例中常见的较长间隙。
Nerve guide conduits (NGCs) with geometric design have shown significant advantages in guidance of nerve reinnervation across the defect of injured peripheral nerves. It is realized that intraluminal fillers with distinctive structure can effectively provide an inner guidance for sprouting of axons and improve the permeability of NGC. In this work, a poly(lactic-co-glycolic acid) (PLGA) NGC is prepared containing intraluminal sponge fillers (labeled as ISF-NGC) and used for reconstruction of a rat sciatic nerve with a 10 mm gap. For comparison, the same procedure is applied to a single hollow PLGA NGC (labeled as H-NGC) and an autologous nerve. As evidenced by significantly improved nerve morphology and function, the ISF-NGC achieves a superior nerve repair effect over H-NGC, which is comparable to autologous nerve grafting. It is likely that the H-NGC only provides a protected tunnel for nerve fiber regrowth and axonal extension, while ISF-NGC offers an extracellular matrix-mimetic architecture as autograft to provide contact guidance for nerve reinnervation. This newly developed ISF-NGC is a promising candidate to aid nerve reinnervation across longer gaps commonly encountered in clinical cases.