Ultrasound backscatter microscope analysis of mouse melanoma progression
Ultrasound backscatter microscope analysis of mouse melanoma progression
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DOI：
10.1016/0301-5629(96)00107-x
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发表时间：
1996-01-01
影响因子：
2.9
通讯作者：
Foster, FS
中科院分区：
文献类型：
--
作者：
Turnbull, DH;Ramsay, JA;Foster, FS
皮肤黑色素瘤的发病率和死亡率在世界范围内持续增加，使得黑色素瘤生物学的研究成为当前研究的重要领域。虽然最近在转基因小鼠技术的突破，导致有前途的黑色素瘤小鼠皮肤模型，目前还没有技术可用于定量研究皮下黑色素瘤的进展，在体内。我们展示了第一个应用程序的成像方法称为超声背散射显微镜（UBM）成像早期小鼠黑色素瘤的空间分辨率为30 μ m的轴向和60 μ m的横向。小鼠B16 F10黑色素瘤从最早的检测开始成像，经过几天，直到它们的直径为2至5 mm。黑色素瘤的尺寸测量UBM被认为是在良好的协议与那些确定的切除肿瘤的组织病理学。UBM确定的黑色素瘤高度和宽度的相对均方根误差分别为8.7%和4.2%。发现早期小鼠黑色素瘤肿瘤高度的平均增加率为0.37 +/- 0.06 mm/天。计算机生成的黑色素瘤的体积渲染已经从三维图像数据中产生，允许对肿瘤体积进行定量比较。使用椭球体肿瘤形状的先验假设，UBM确定的体积的相对误差小于17%。这些结果应该是相当大的兴趣，研究黑色素瘤生物学使用小鼠皮肤模型，并在使用高频超声成像皮肤黑色素瘤的临床评估的影响。
The incidence and mortality rate of cutaneous melanoma continue to increase throughout the world, making the study of melanoma biology an important area of current research. While recent breakthroughs in transgenic mouse technology have led to promising mouse skin models of melanoma, there is presently no technique available for quantitatively studying subsurface melanoma progression, in vivo. We demonstrate the first application of an imaging method called ultrasound backscatter microscopy (UBM) for imaging early murine melanomas with spatial resolution of 30 mu m axial and 60 mu m lateral. Murine B16 F10 melanomas have been imaged from their earliest detection, over several days, until they are 2 to 5 mm in diameter. Melanoma dimensions measured by UBM were found to be in excellent agreement with those determined histopathologically on the excised tumours. The relative rms errors in UBM-determined melanoma height and width were found to be 8.7% and 4.2%, respectively. The mean rate of increase in tumour height of early murine melanoma was found to be 0.37 +/- 0.06 mm/day. Computer-generated volumetric renderings of melanomas have been produced from three-dimensional image data, allowing quantitative comparisons of tumour volumes to be made. Using a priori assumptions of ellipsoid tumour shape, the relative error in UBM-determined volume was shown to be less than 17%. These results should be of considerable interest to investigators studying melanoma biology using mouse skin models, and have implications in the use of high frequency ultrasound imaging for the clinical assessment of cutaneous melanoma.