A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation.
A genetic analysis of Candida albicans: isolation of a wide variety of auxotrophs and demonstration of linkage and complementation.
复制标题
白色念珠菌的遗传分析：多种营养缺陷型的分离以及连锁和互补的证明。
DOI：
10.1093/genetics/104.2.241
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发表时间：
1983
期刊：
影响因子：
3.3
通讯作者：
Magee,PT
中科院分区：
文献类型：
--
作者：
Kakar,SN;Partridge,RM;Magee,PT
天然存在的白色念珠菌菌株对于有限数量的营养标记来说似乎是二倍体和杂合的。额外的杂合性可以通过诱变剂处理来诱导；就产生多种突变的功效和效率而言，单独的亚硝酸或与紫外线组合是一种有效的诱变剂。原生质球融合，然后在选择性培养基上再生，揭示了所分析的四种需要组氨酸的突变体之间的互补性。一些融合产物似乎是稳定的原养生物，而在其他融合产物中则产生了几种分离子，显然是由于染色体或核消除所致。结果表明存在异核现象和核融合。所描述的程序可成功用于生成新突变体和研究等位性。三组连锁关系源自几个营养缺陷型标记的同时出现或共分离提供的证据。
Naturally occurring strains ofCandida albicansappear to be diploid and heterozygous for a limited number of nutritional markers. Additional heterozygosity can be induced by treatment with mutagens; nitrous acid alone or in combination with UV is a potent mutagen in terms of both efficacy and efficiency in the production of a wide variety of mutations. Spheroplast fusion followed by regeneration on selective media revealed complementation among four histidine-requiring mutants analyzed. Some of the fusion products appeared to be stable prototrophs, whereas in others several kinds of segregants resulted, apparently due to chromosomal or nuclear elimination. The results are suggestive of both heterokaryosis as well as nuclear fusion. The procedures described can be successfully used for generating new mutants and studying allelism. Three sets of linkage relationships have been derived from evidence provided by concomitant appearance or cosegragation of several auxotrophic markers.