Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae.
Virion DNA-independent RNA polymerase from Saccharomyces cerevisiae.
复制标题
来自酿酒酵母的病毒颗粒 DNA 独立的 RNA 聚合酶。
DOI：
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发表时间：
1980
影响因子：
14.9
通讯作者：
Reed B. Wicknert
中科院分区：
文献类型：
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作者：
J.Douglas Welsh;Michael J. Leibowitz;Reed B. Wicknert
细胞内病毒颗粒中存在酵母的“杀伤”质粒和较大的双链RNA质粒。从酵母的二倍体杀伤菌株（在乙醇上生长成稳定生长）纯化这些颗粒导致DNA非依赖性RNA聚合酶活性的共纯化。该活性结合并需要所有四种核糖核苷三磷酸，并且不会作用于脱氧核糖核苷三磷酸。该反应需要镁，被巯基氧化剂和高浓度的单价阳离子抑制，但对DNA酶、α-鹅膏蕈碱和放线菌素D不敏感。焦磷酸盐和溴化乙锭一样抑制反应。外源核酸对反应无影响。产物主要是单链RNA，其中一些是从酶活性病毒体释放的。
The "killer" plasmid and a larger double-stranded RNA plasmid of yeast exist in intracellular virion particles. Purification of these particles from a diploid killer strain of yeast (grown into stationary growth on ethanol) resulted in co-purification of a DNA-independent RNA polymerase activity. This activity incorporates and requires all four ribonucleoside triphosphates and will not act on deoxyribonucleoside triphosphates. The reaction requires magnesium, is inhibited by sulfhydryl-oxidizing reagents and high concentrations of monovalent cation, but is insensitive to DNase, alpha-amanitin, and actinomycin D. Pyrophosphate inhibits the reaction as does ethidium bromide. Exogenous nucleic acids have no effect on the reaction. The product is mostly single-stranded RNA, some of which is released from the enzymatically active virions.