Establishment of four new mesothelioma cell lines: characterization by ultrastructural and immunophenotypic analysis
Establishment of four new mesothelioma cell lines: characterization by ultrastructural and immunophenotypic analysis
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DOI：
10.1183/09031936.99.13352799
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发表时间：
1999-03-01
影响因子：
24.3
通讯作者：
Mutti, L
中科院分区：
文献类型：
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作者：
Orengo, AM;Procopio, A;Mutti, L
本研究的目的是评估四个新的恶性间皮瘤（MM）细胞系的生物学特性。由于最近在MM中检测到猴病毒（SV）40序列，因此还分析了SV 40大T抗原（Tag）的表达，并通过形态学、超微结构和细胞遗传学分析对MM细胞系进行了表征。Tag和相关MM标志物的表达通过免疫细胞化学、表面抗原通过间接免疫荧光和免疫调节细胞因子通过酶联免疫吸附试验（ELISA）进行了研究。细胞系表达波形蛋白、细胞角蛋白8和18以及HBME-1单克隆抗体识别的间皮抗原，但不表达癌胚抗原。表面人类白细胞抗原（HLA）-I类和细胞间粘附分子（ICAM）-1分子存在于所有的细胞系。虽然HLA II类和CD 86是组成型不可检测的，但在干扰素（IFN）-γ刺激后存在HLA II类。所有细胞系均显示出染色体6异常的异常核型，转化生长因子（TGF）-β（2）和白细胞介素（IL）-6是组成型分泌的，而肿瘤坏死因子（TNF）-α仅在对脂多糖的反应中分泌，核内Tag在两种细胞系中表达，大T抗原与人类白细胞抗原I类和细胞间粘附分子1阳性的持续存在可能表明大T抗原作为某些恶性间皮瘤患者细胞毒性T淋巴细胞免疫治疗的靶点。
The aim of this study was to assess the biological characteristics of four new malignant mesothelioma (MM) cell lines. Since simian virus (SV)40 sequences have been recently detected in MM, SV40 large T antigen (Tag) expression was also analysed,MM cell lines were characterized by morphological, ultrastructural and cytogenetic analysis. Expression of Tag and of relevant MM markers was studied by immunocytochemistry, surface antigens by indirect immunofluorescence and immunomodulating cytokines by enzyme-linked immunosorbent assay (ELISA).The four MM cell lines, established from pleural effusions, showed a slow proliferation rate and pleomorphic changes during culture. Cell lines expressed vimentin, cytokeratins 8 and 18, and the mesothelial antigen recognized by HBME-1 monoclonal antibody, but not carcinoembryonic antigen. Surface human leukocyte antigen (HLA)-class I and intercellular adhesion molecule (ICAM)-1 molecules were present on all the cell lines. While HLA class II and CD86 were constitutively undetectable, HLA-class II was present after interferon (IFN)-gamma stimulation, All cell lines displayed abnormal karyotypes with chromosome 6 abnormalities, Transforming growth factor (TGF)-beta(2) and interleukin (IL)-6 were constitutively secreted, while tumour necrosis factor (TNF)-alpha was secreted only in response to lipopolysaccharide, Intranuclear Tag was expressed in two cell lines.The persistence of large T antigen with human leukocyte antigen class I and intercellular adhesion molecule-1 positivity may point to large T antigen as a target for cytotoxic T-lymphocyte-based immunotherapy in some malignant mesothelioma patients.