Functional analysis of an established mouse vascular endothelial cell line
Functional analysis of an established mouse vascular endothelial cell line
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DOI：
10.1159/000098520
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发表时间：
2007-01-01
影响因子：
1.7
通讯作者：
Saito, Ichiro
中科院分区：
文献类型：
--
作者：
Nishiyama, Tatsuaki;Mishima, Kenji;Saito, Ichiro
背景：使用细胞系的体外研究对于理解细胞机制是有用的。本研究的目的是建立一个新的永生化主动脉血管内皮细胞（EC）系，保留内皮细胞的特性，可以促进EC的研究。方法：从p53基因缺陷的小鼠主动脉建立小鼠主动脉血管内皮细胞系（MAEC），培养100代以上。评估内皮标志物的表达，并通过管形成和结合试验分析该细胞系的功能。结果如下：MAEC保留了许多内皮特性，如鹅卵石外观，接触抑制生长，乙酰化低密度脂蛋白的主动摄取，存在的韦伯-帕拉德体和几个EC标志物。MAECs在体外和体内均表现出管形成活性。此外，至关重要的是，肿瘤坏死因子α，一种炎症细胞因子，促进淋巴细胞粘附MAECs，这表明MAECs可能有助于动脉粥样硬化和局部炎症反应的体外研究。结论：我们描述了MAEC的形态学和细胞生物学特性，提供了强有力的证据，它保留了内皮特性。这种新的细胞系可以成为研究EC生物学的有用工具。版权所有（c）2007 S. Karger AG，巴塞尔
Background: In vitro studies using cell lines are useful for the understanding of cellular mechanisms. The purpose of our study is to develop a new immortalized aortic vascular endothelial cell (EC) line that retains endothelial characteristics and can facilitate the study of ECs. Methods: A mouse aortic vascular EC line (MAEC) was established from p53-deficient mouse aorta and cultured for over 100 passages. The expression of endothelial markers was assessed, and the function of this cell line was analyzed by tube formation and binding assays. Results: MAEC retained many endothelial properties such as cobblestone appearance, contact-inhibited growth, active uptake of acetylated low-density lipoprotein, existence of Weibel-Palade bodies and several EC markers. MAECs exhibited tube formation activity both in vitro and in vivo. Furthermore, crucially, tumor necrosis factor alpha, an inflammatory cytokine, promoted lymphocyte adhesion to MAECs, suggesting that MAECs may facilitate the study of atherosclerosis and local inflammatory reactions in vitro. Conclusion: We describe the morphological and cell biological characteristics of MAEC, providing strong evidence that it retained endothelial properties. This novel cell line can be a useful tool for studying the biology of ECs. Copyright (c) 2007 S. Karger AG, Basel