Untargeted metabolomics coupled with chemometric analysis reveals species-specific steroidal alkaloids for the authentication of medicinal Fritillariae Bulbus and relevant products
Untargeted metabolomics coupled with chemometric analysis reveals species-specific steroidal alkaloids for the authentication of medicinal Fritillariae Bulbus and relevant products
复制标题
非靶向代谢组学结合化学计量分析揭示物种特异性甾体生物碱，用于药用贝母及相关产品的鉴定
DOI：
10.1016/j.chroma.2019.460630
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发表时间：
2020-02-08
影响因子：
4.1
通讯作者：
Li, Hui-Jun
中科院分区：
文献类型：
--
作者：
Liu, Feng-Jie;Jiang, Yan;Li, Hui-Jun
草药的质量控制体系中包含了对原始品种的认证。本研究采用超高效液相色谱-四极杆飞行时间质谱联用非靶向代谢组学与化学计量学分析相结合的方法，对贝母的原料和商业产品进行了精确鉴定。首先，提出了8种药用贝母的逐步差异放大化学计量分析策略。随后，发现了21个种特异性标记，并对不同制样方法下的特异性进行了研究。最后，利用所获得的物种特异性标记成功地鉴定了贝母在商业相关产品中的种类。这项工作首次报告了用于贝母产品认证的稳健和特定标记，显示了跟踪草药供应商供应链的希望。(C) 2019 Elsevier B.V.版权所有
Authentication of original species is embedded in the quality control system of herbal medicines. In this work, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-based untargeted metabolomics coupled with chemometric analysis was utilized for the precise authentication of the Fritillaria species for both raw materials and commercial products. First, a stepwise difference enlarging chemometric analysis strategy was proposed to analyze eight medicinal Fritillaria species. Subsequently, 21 species-specific markers were discovered and the specificity was investigated under different sample preparation methods. Finally, the obtained species-specific markers were successfully utilized to identify the Fritillaria species in commercially relevant products. This work is the first to report robust and specific markers for authentication of Fritillaria products, showing promise for tracking the supply chain of herbal suppliers. (C) 2019 Elsevier B.V. All rights reserved.