The influence of storage conditions on the clonogenic capacity of periodontal ligament cells: implications for tooth replantation.
The influence of storage conditions on the clonogenic capacity of periodontal ligament cells: implications for tooth replantation.
复制标题
储存条件对牙周膜细胞克隆形成能力的影响：对牙齿再植的影响。
DOI：
10.1046/j.1365-2591.1998.00138.x
复制
发表时间：
1998
影响因子：
5
通讯作者：
E. Barrett
中科院分区：
文献类型：
--
作者：
P. Lekic;D. Kenny;E. Barrett
牙周韧带细胞的生长发育是牙再植后牙周韧带修复的必要条件。如果不能立即再植，可以通过防止干燥和在生理介质中储存来延长PL祖细胞的繁殖能力（克隆生成能力）和重新定植在伤口上。本研究考察了唾液、牛奶、汉克平衡盐溶液（HBSS）和Eagle培养基（α - MEM）对人PL祖细胞在肺泡外时间30和60 min时克隆生成能力的影响。在本研究中，使用了20颗萌出的人类前磨牙，尽可能以无损伤的方式拔出，用于正畸目的。15颗前磨牙立即置于刚采集的自体唾液中，室温（+ 23℃）放置15分钟。接下来，这15颗前磨牙被分成三组，每组5颗，分别储存在唾液、牛奶或HBSS中，温度为+ 4摄氏度，并被冰包围的塑料杯中。剩余的5颗牙齿作为阳性对照，立即置于+ 4℃的α MEM中，在30和60分钟的总牙槽外时间内，用手术刀从一半的根表面刮下PL组织。通过30分钟的酶解程序将细胞从组织样本中释放出来，并对组织样本中的细胞进行克隆生成能力分析。随着时间的推移，所有方案的克隆生成能力都有所下降。在α - MEM中储存的牙周膜细胞在30 ~ 60分钟内减少最少，而在唾液中储存的PL细胞减少最多。从唾液转移到牛奶或HBSS后的克隆生成能力在30分钟内差异不显著。60 min时，从唾液转移到HBSS的细胞克隆细胞百分比高于转移到牛奶中的细胞（5.9% vs. 3.5%, P < 0.05）。我们的结论是，将撕脱的牙齿立即储存在自体唾液中，然后转移到冷冻牛奶中，可以保留足量的前体细胞，以保证再植根，并在牙槽外持续60分钟的时间内实现牙槽外愈合。
Viable periodontal ligament (PL) cells are required for PL healing of avulsed teeth following replantation. If immediate replantation cannot be accomplished, the ability of PL progenitor cells to reproduce (clonogenic capacity) and recolonize the wound may be extended by prevention of desiccation and storage in physiological media. This investigation examined the effects of storage in saliva, milk, Hank's balanced salt solution (HBSS) and Eagle's medium (alpha MEM) on the clonogenic capacity of human PL progenitor cells at 30 and 60 min extra-alveolar time. Twenty erupted human premolar teeth extracted as atraumatically as possible for orthodontic purposes were used in the present study. Fifteen premolars were placed immediately in freshly collected autologous saliva at room temperature, (+ 23 degrees C) for 15 min. These 15 premolars were next divided into three groups of five and stored in either saliva, milk or HBSS at + 4 degrees C in plastic cups surrounded by ice. The remaining five teeth served as positive controls and were immediately placed in alpha MEM at + 4 degrees C. PL tissue was scraped from one-half of the root surface with a scalpel at 30 and 60 min total extra-alveolar duration. Cells were released from the tissue sample with a 30 min enzymatic digestion procedure and the cells from the tissue samples analyzed for clonogenic capacity. There was a reduction in clonogenic capacity with time for all protocols. Periodontal ligament cells stored in alpha MEM showed the least reduction between 30 and 60 min and the greatest reduction was observed for PL cells stored in saliva. The difference in clonogenic capacity following transfer from saliva to milk or HBSS was not significant at 30 min. At 60 min, cells transferred from saliva to HBSS had a statistically higher percentage of clonogenic cells than those transferred to milk (5.9% vs. 3.5%; P < 0.05). We conclude that immediate storage of avulsed teeth in autologous saliva, followed by transfer to chilled milk, preserves the presence of sufficient progenitor cells in the PL to warrant replantation and the possibility of PL healing at 60 min extra-alveolar duration.