AN ALTERNATIVE PATHWAY FOR TRANSCRIPTION INITIATION INVOLVING TFII-I
AN ALTERNATIVE PATHWAY FOR TRANSCRIPTION INITIATION INVOLVING TFII-I
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DOI：
10.1038/365355a0
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发表时间：
1993-09-23
期刊：
影响因子：
64.8
通讯作者：
ROEDER, RG
中科院分区：
文献类型：
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作者：
ROY, AL;MALIK, S;ROEDER, RG
RNA聚合酶II启动所需的最小启动子元件包括TATA盒和/或转录起始位点处或附近的启动子元件（Inr）1 -4。对腺病毒主要晚期核心启动子（含有两种元件）的研究已经证明了涉及转录因子TFIID（或衍生的亚基，TATA结合蛋白TBP（TFIIDtau））与TATA元件的结合的起始途径，其由转录因子TFIIA促进，随后是其他一般因子的顺序相互作用4 -8。在这里，我们描述了一个新的途径，需要一个完整的Inr和Inr结合因子TFII-I（参考文献3）。顺序添加的一般因素产生的TFII-I依赖的preinitiation复合物不同于与TFIIA形成的。此外，TBP（仅与TFII-I）协同结合至含Inr的TATA较少的启动子，这表明了用于活化TATA较少的启动子的手段，尽管如此，TATA较少的启动子需要TFIID（参考文献9-11）。这些观察结果提供了支持功能不同的途径，可能会受到特定的激活剂或抑制剂的差异调节。
THE minimal promoter elements required for initiation by RNA polymerase II include the TATA box and/or an initiator element (Inr) at or near the transcription start site1-4. Studies of the adeno-virus major late core promoter (containing both elements) have demonstrated an initiation pathway that involves binding of the transcription factor TFIID (or the derived subunit, the TATA-binding protein TBP (TFIIDtau)) to the TATA element, which is facilitated by transcription factor TFIIA, followed by sequential interactions of other general factors4-8. Here we describe a novel pathway that requires an intact Inr and the Inr-binding factor TFII-I (ref. 3). Sequential addition of the general factors generated TFII-I-dependent preinitiation complexes different from those formed with TFIIA. Furthermore, TBP bound cooperatively (with only TFII-I) to an Inr-containing TATA-less promoter, suggesting a means for activation of TATA-less promoters, which nonetheless require TFIID (refs 9-11). These observations provide support for functionally distinct pathways which could be subject to differential regulation by specific activators or repressors.