High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
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DOI：
10.1038/nprot.2007.13
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Schiestl, Robert H.
中科院分区：
文献类型：
--
作者：
Gietz, R. Daniel;Schiestl, Robert H.
在这里，我们描述了一种高效的醋酸锂/单链载体DNA/聚乙二醇法转化酿酒酵母。这种方法目前提供了转化子的最高效率和产量，尽管有一种更快的方案可用于少量的转化。酵母培养完成后，根据热休克的时间长短，这一过程需要长达1.5小时。此方法对于大多数转换要求都很有用。
Here we describe a high-efficiency version of the lithium acetate/single-stranded carrier DNA/PEG method of transformation of Saccharomyces cerevisiae. This method currently gives the highest efficiency and yield of transformants, although a faster protocol is available for small number of transformations. The procedure takes up to 1.5 h, depending on the length of heat shock, once the yeast culture has been grown. This method is useful for most transformation requirements.