DISINTEGRATION AND RECONSTITUTION OF PHOTOSYSTEM-II REACTION CENTER CORE COMPLEX .1. PREPARATION AND CHARACTERIZATION OF 3 DIFFERENT TYPES OF SUBCOMPLEX
DISINTEGRATION AND RECONSTITUTION OF PHOTOSYSTEM-II REACTION CENTER CORE COMPLEX .1. PREPARATION AND CHARACTERIZATION OF 3 DIFFERENT TYPES OF SUBCOMPLEX
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DOI：
10.1016/0005-2728(88)90170-3
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发表时间：
1988-03-09
期刊：
影响因子：
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通讯作者：
TOYOSHIMA, Y
中科院分区：
文献类型：
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作者：
AKABORI, K;TSUKAMOTO, H;TOYOSHIMA, Y
用温和的洗涤剂从菠菜PS II中分离光系统II （PS II）反应中心核心复合物（RC复合物），成功分离出3种亚复合物（配合物1-3），保留了它们的假基和两个分子质量分别为47 kDa和43 kDa的色素结合亚基多肽。复合物1多肽组成为47 kDa/43 kDa/D1细胞色素b-559，复合物2多肽组成为47 kDa/D1/D2细胞色素b-559，复合物3多肽组成为D1/D2/细胞色素b-559。复合体3被证实含有4个Chl a， 2个叶绿素a， 1个β。-胡萝卜素和一个质体醌A-9 （PQA）的摩尔比。除了PQA外，这些数字与Nanba和Satoh报告的D1/D2/细胞色素b-559颗粒的结果基本相同。本研究分离的三个亚复合物至少保留了一个PQA。然而，在这三种制备中，P-680与QA之间并没有光诱导电荷分离，而是光诱导P-680+与Pheo a复合形成了三重态P-680。三重态的衰变时间为25 μ s。有证据表明，在类囊体脂和类囊体脂加PQA的复合物1和复合物2中，QA功能进行了重构。
Disintegration of the Photosystem II (PS II) reaction center core complex (RC complex) from the spinach PS II by using mild detergents resulted in success of isolating three types of subcomplex (complexes 1-3), retaining their prosthetic groups and two pigments-binding subunit polypeptides with molecular masses of 47 kDa and 43 kDa. The polypeptide compositions of the three complexes isolated were 47 kDa/43 kDa/D1 cytochrome b-559 in complex 1, 47 kDa/D1/D2-cytochrome b-559 in complex 2 and D1/D2/cytochrome b-559 in complex 3. The complex 3 was confirmed to have four Chl a, two pheophytins a, one .beta.-carotene and one plastoquinone A-9 (PQA) in molar ratio. These numbers were essentially the same as in the results reported by Nanba and Satoh on their D1/D2/cytochrome b-559 particle, except for PQA. All of the three subcomplexes isolated in the present work preserved one PQA at least. However, instead of the photoinduced charge separation between P-680 and QA the formation of the triplet state P-680 as a result of recombination of photoinduced P-680+ and Pheo a was observed in the three preparations. The decay time of the triplet state was determined as 25 .mu.s. Evidence suggesting that the reconstitution of QA function was performed in complex 1 and complex 2 with the thylakoid lipids and thylakoid lipids plus PQA, respectively, was obtained.