Assessment of fiberoptic near-infrared Raman spectroscopy for diagnosis of bladder and prostate cancer
Assessment of fiberoptic near-infrared Raman spectroscopy for diagnosis of bladder and prostate cancer
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DOI：
10.1016/j.urology.2004.12.058
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发表时间：
2005-06-01
期刊：
影响因子：
2.1
通讯作者：
Wongkeesong, LM
中科院分区：
文献类型：
--
作者：
Crow, P;Molckovsky, A;Wongkeesong, LM
目标。为了确定适合体内使用的光纤拉曼系统是否能够在体外区分良性和恶性膀胱癌和前列腺癌病理结果。拉曼光谱是一种光学技术，它通过分析组织散射激光的方式来测量组织的分子组成。实验室研究表明，该技术可用于体外鉴定移行细胞癌和前列腺癌。从29例膀胱镜手术中采集的冰冻膀胱标本中记录了220个拉曼光谱，从38例经尿道前列腺电切术中采集的冰冻前列腺标本中记录到197个拉曼光谱。光谱与组织学特征相关，并用于构建针对膀胱和前列腺的单独诊断算法。对这些算法进行了测试，以确定从拉曼光谱中确定样本的病理发现的能力。BUBLE算法能够区分良性样本(正常和膀胱炎)和恶性样本(移行细胞癌)，总体准确率为84%。该算法能够区分良性样本(良性前列腺增生症和前列腺炎)和恶性样本(前列腺癌)，总体准确率为86%。本研究结果表明，临床拉曼系统可以为前列腺癌和膀胱癌的体外诊断提供一种准确、客观的方法。由于拉曼探针适合在内窥镜、腹腔镜或开放手术中使用，这项工作为体内研究铺平了道路。
Objectives. To determine whether a fiberoptic Raman system, suitable for in vivo use, is able to differentiate between benign and malignant bladder and prostate pathologic findings in vitro. Raman spectroscopy is an optical technique that provides a measure of the molecular composition of tissue by analyzing the way that tissue scatters laser light. Laboratory studies have shown that the technique can be used to identify and characterize transitional cell carcinoma and prostate adenocarcinoma in vitro.Methods. A total of 220 Raman spectra were recorded from 29 snap-frozen bladder samples collected at cystoscopic procedures, and 197 Raman spectra were recorded from 38 snap-frozen prostate samples collected at transurethral resection of the prostate. The spectra were correlated with the histologic features and used to construct separate diagnostic algorithms for the bladder and prostate. These algorithms were tested as to their ability to determine the pathologic finding of a sample from its Raman spectrum.Results. The bladder algorithm was able to differentiate benign samples (normal and cystitis) from malignant samples (transitional cell carcinoma), with an overall accuracy of 84%. The prostate algorithm was able to differentiate benign samples (benign prostatic hyperplasia and prostatitis) from malignant samples (prostate cancer), with an overall accuracy of 86%.Conclusions. The results of this study have demonstrated that the clinical Raman system can provide an accurate and objective method to diagnose prostate and bladder cancer in vitro. Because the Raman probe is suitable for use during endoscopic, laparoscopic, or open procedures, this work paves the way for in vivo studies.