Three-dimensional printed electrospun fiber-based scaffold for cartilage regeneration
Three-dimensional printed electrospun fiber-based scaffold for cartilage regeneration
复制标题
用于软骨再生的三维打印静电纺丝纤维支架
DOI：
10.1016/j.matdes.2019.107886
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发表时间：
2019-10-05
影响因子：
8.4
通讯作者：
Zhou, Guangdong
中科院分区：
文献类型：
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作者：
Chen, Weiming;Xu, Yong;Zhou, Guangdong
制备具有可定制形状和大孔的三维（3D）静电纺丝纤维支架是一项具有挑战性的任务。在这项研究中，首次将一维（1D）明胶/聚（乳酸-乙醇酸共聚物）（PLGA）电纺纤维加工成适合3D打印的墨水。通过将3D打印和冷冻干燥相结合，电纺纤维基墨水成功地制造成3D打印支架（3DP），除了具有与天然细胞外基质（ECM）相似的纤维表面形态外，还具有精确控制的形状和大孔。3DP表现出良好的弹性和水诱导的形状记忆，并且被发现上级使用纯明胶纤维制造的3D打印支架和使用非纤维明胶/PLGA粉末制造的冷冻成形支架。此外，3DP与软骨细胞结合在体内获得了令人满意的软骨再生。本研究中建立的3D打印电纺纤维的新策略为各种组织再生应用的多种支架的设计和制造提供了研究模型。(c)2019年，任作家。由爱思唯尔有限公司出版。这是一篇在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
The fabrication of three-dimensional (3D) electrospun fibrous scaffolds with customizable shapes and large pores is a challenging task. In this study, for the first time, one-dimensional (1D) gelatin/poly (lactic-co-glycolic acid) (PLGA) electrospun fibers were processed into inks suitable for 3D printing. By combining 3D printing and freeze drying, electrospun fiber-based inks were successfully fabricated into 3D-printed scaffolds (3DP) with precisely controlled shapes and large pores, in addition to fibrous surface morphologies similar to that of a native extracellular matrix (ECM). The 3DP exhibited good elasticity and water-induced shape memory, and was found to be superior to 3D-printed scaffolds fabricated using pure gelatin fibers and freeze-shaped scaffolds fabricated using non-fibrous gelatin/PLGA powder. Moreover, 3DP combined with chondrocytes achieved satisfactory cartilage regeneration in vivo. The novel strategy of 3D printing electrospun fibers established in this study provides a research model for the design and fabrication of multiple scaffolds for various tissue regeneration applications. (c) 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).