Rapid visualization of protein--dodecyl sulfate complexes in polyacrylamide gels.
Rapid visualization of protein--dodecyl sulfate complexes in polyacrylamide gels.
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DOI：
10.1016/0003-2697(76)90202-5
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发表时间：
1976-06
影响因子：
2.9
通讯作者：
L. Nelles;J. R. Bamburg
中科院分区：
文献类型：
--
作者：
L. Nelles;J. R. Bamburg
建立了一种快速、灵敏的含十二烷基硫酸钠（SDS）的聚丙烯酰胺凝胶上蛋白质条带的可视化和定量技术。可视化是基于与蛋白质结合的不溶性月桂基硫酸钾盐的形成。由此形成的沉淀物在几分钟内可见，低至0.06 μg蛋白质/mm 2凝胶横截表面积的水平，并且在清除凝胶背景后，可以通过光密度测定法定量含有0.12至1.5 μg蛋白质/mm 2凝胶横截表面积的条带。该技术已被应用于肌肉肌动蛋白，脑微管蛋白，卵清蛋白，给出相同的标准曲线。
A rapid and sensitive technique for the visualization and quantitation of protein bands on sodium dodecyl sulfate (SDS)-containing polyacrylamide gels has been developed. Visualization is based on the formation of the insoluble potassium salt of lauryl sulfate bound to the protein. Precipitates thus formed are visible within minutes down to the level of 0.06 μg of protein/mm2of gel cross-sectional surface area, and bands containing from 0.12 to 1.5 μg of protein/mm2of gel cross-sectional surface area may be quantitated by densitometry following clearing of the gel background. The technique has been applied to muscle actin, brain tubulin, and ovalbumin which give identical standard curves.