Connective tissue fibroblast response to acupuncture: Dose-dependent effect of bidirectional needle rotation
Connective tissue fibroblast response to acupuncture: Dose-dependent effect of bidirectional needle rotation
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DOI：
10.1089/acm.2007.6351
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发表时间：
2007-04-01
影响因子：
2.6
通讯作者：
Badger, Gary J.
中科院分区：
文献类型：
--
作者：
Langevin, Helene M.;Bouffard, Nicole A.;Badger, Gary J.
背景：虽然针灸手法是针灸疗法的重要组成部分，但目前关于特定类型的针灸手法是否会对人体产生不同的影响的信息很少。双向(来回)旋转是针灸实践中最常见的针灸手法之一。目的：本研究假设双向针灸旋转引起结缔组织成纤维细胞活跃的细胞骨架重塑，其作用类似于先前的单向旋转。干预：来自18只小鼠的皮下组织被随机分为不同的双向旋转周期(8-)和旋转周期幅度(180度-720度)体外30分钟，然后进行组织固定，共聚焦显微镜，并测量成纤维细胞胞体横截面积。结果：与单向旋转一样，成纤维细胞对双向旋转的反应是广泛的细胞扩散和片状脂膜的形成。双向针旋转对成纤维细胞胞体横截面积有显著的整体影响(方差分析，p<；0.001)。细胞对双向旋转的反应是非单调的，最大反应出现在特定的刺激窗口内，周期幅度和周期数。结论：这些发现表明，针灸手法的细微差异可以影响小鼠皮下结缔组织的细胞反应。还需要进一步的研究来确定这些结缔组织反应是否与治疗效果有关。
Background: Although acupuncture-needle manipulation is an important component of acupuncture therapy, little information is currently available on whether or not specific types of needle manipulation produce different effects on the body. Bidirectional (back-and-forth) rotation is one of the most common forms of needle manipulation used in acupuncture practice.Objectives: In this study, we hypothesized that bidirectional acupuncture needle rotation causes dose-dependent active cytoskeletal remodeling in connective tissue fibroblasts similar to that previously demonstrated with unidirectional rotation.Interventions: Subcutaneous tissue explants from 18 mice were randomized to varying amounts of bidirectional rotation cycles (8-64) and rotation-cycle amplitude (180 degrees-720 degrees) ex vivo for 30 minutes, followed by tissue fixation, confocal microscopy, and measurement of fibroblast cell body cross-sectional area.Results: As with unidirectional rotation, fibroblasts responded to bidirectional rotation with extensive cell spreading and lamellipodia formation. Bidirectional needle rotation had a significant overall effect on fibroblast cell body cross sectional area (analysis of variance, p < 0.001). The cellular response to bidirectional rotation was nonmonotonic with maximal responses occurring within specific stimulus windows with regard to cycle amplitude and cycle number.Conclusions: These findings demonstrate that subtle differences in acupuncture-needle manipulation techniques can affect cellular responses in mouse subcutaneous connective tissue. Further studies will be needed to determine whether these connective-tissue responses are related to therapeutic effects.