Urodynamic function during sleep-like brain states in urethane anesthetized rats.
Urodynamic function during sleep-like brain states in urethane anesthetized rats.
复制标题
DOI：
10.1016/j.neuroscience.2015.11.027
复制
发表时间：
2016-01-28
期刊：
影响因子：
3.3
通讯作者：
Lovick T
中科院分区：
文献类型：
--
作者：
Crook J;Lovick T
麻醉大鼠脑电出现类睡眠状态的自发变化。排尿阈值在慢波睡眠样状态期间增加。在慢波EEG状态下，PAG神经元的排尿相关活动减少。睡眠样EEG状态的变化可能会影响身体系统的PAG介导的控制。目的是研究尿流动力学参数和中脑导水管周围灰质（PAG）的功能兴奋性在睡眠样脑状态的变化在乌拉坦麻醉大鼠。同时记录逼尿肌压力，尿道外括约肌（EUS）肌电图（EMG），皮层脑电图（EEG），并在PAG的单单位活动在重复排尿诱导连续注入生理盐水到膀胱。EEG在同步的高振幅慢波活动（SWA）和类似于慢波和“激活的”睡眠样大脑状态的去干扰的低振幅快活动之间循环。在（SWA，0.5- 1.5Hz同步振荡的EEG波形）排尿变得比“激活”脑状态（2-5 Hz低振幅去振荡的EEG波形）更不规则，逼尿肌排尿压力阈值、排尿量阈值和尿道外括约肌EMG爆发活动的持续时间提高。尾侧PAG和邻近被盖内记录的23/52个神经元的自发放电率与EEG状态有关，大多数反应细胞（92%）在SWA期间放电更慢。几乎四分之一的记录细胞（12/52）显示出与空隙发生相关的放电频率的阶段性变化。观察到抑制（n = 6）、兴奋（n = 4）或兴奋/抑制（n = 2）。83%的排尿反应细胞的自发放电率对EEG状态的变化敏感。在12个响应细胞中的9个（75%）中，在SWA期间响应降低。我们建议，在不同的睡眠样脑状态的尿动力学特性的变化发生，这可能与改变兴奋性的排尿电路在导水管周围灰质。
Spontaneous changes in sleep-like EEG state occur in urethane-anesthetized rats. The micturition threshold is increased during the slow wave sleep-like state. Micturition-related activity of PAG neurons was reduced during slow wave EEG states. Changes in sleep-like EEG state may impact PAG-mediated control of bodily systems. The aim was to investigate urodynamic parameters and functional excitability of the periaqueductal gray matter (PAG) during changes in sleep-like brain states in urethane anesthetized rats. Simultaneous recordings of detrusor pressure, external urethral sphincter (EUS) electromyogram (EMG), cortical electroencephalogram (EEG), and single-unit activity in the PAG were made during repeated voiding induced by continuous infusion of saline into the bladder. The EEG cycled between synchronized, high-amplitude slow wave activity (SWA) and desynchronized low-amplitude fast activity similar to slow wave and ‘activated’ sleep-like brain states. During (SWA, 0.5–1.5 Hz synchronized oscillation of the EEG waveform) voiding became more irregular than in the ‘activated’ brain state (2–5 Hz low-amplitude desynchronized EEG waveform) and detrusor void pressure threshold, void volume threshold and the duration of bursting activity in the external urethral sphincter EMG were raised. The spontaneous firing rate of 23/52 neurons recorded within the caudal PAG and adjacent tegmentum was linked to the EEG state, with the majority of responsive cells (92%) firing more slowly during SWA. Almost a quarter of the cells recorded (12/52) showed phasic changes in firing rate that were linked to the occurrence of voids. Inhibition (n = 6), excitation (n = 4) or excitation/inhibition (n = 2) was seen. The spontaneous firing rate of 83% of the micturition-responsive cells was sensitive to changes in EEG state. In nine of the 12 responsive cells (75%) the responses were reduced during SWA. We propose that during different sleep-like brain states changes in urodynamic properties occur which may be linked to changing excitability of the micturition circuitry in the periaqueductal gray.