Combined Effect of 60 Hz Magnetic Fields and Anticancer Drugs on Human Hepatoma HepG2 Cells
Combined Effect of 60 Hz Magnetic Fields and Anticancer Drugs on Human Hepatoma HepG2 Cells
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60 Hz磁场与抗癌药物对人肝癌HepG2细胞的联合作用
DOI：
10.1109/jerm.2018.2880341
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发表时间：
2019
影响因子：
--
通讯作者：
Yamada S.
中科院分区：
文献类型：
--
作者：
Kakikawa M;Maeda T;Yamada S.
我们以前使用细菌细胞大肠杆菌的数据表明，60 Hz，50 mT的磁场增强了抗生素的功效。通过在临床癌症化疗中使用磁场，推测能够增强抗癌药物对目标区域的功效，减少剂量并抑制副作用。然而，由于原核细菌细胞，即，大肠杆菌与人类细胞不同，人类细胞在许多方面是真核生物和多细胞生物，目前尚不清楚磁场是否影响抗癌药物对人类癌细胞的疗效。本研究设计并制作了体外培养人肝癌细胞的磁场暴露装置，研究了60 Hz、50 mT磁场对抗癌药物对人肝癌HepG2细胞的杀伤作用。抗癌药顺铂的实验结果表明，与单独使用顺铂相比，通过顺铂和磁场的组合，活HepG2细胞的数量显著减少。这表明，60 Hz，50 mT的磁场增加顺铂对人肝癌细胞的杀伤活性。抗癌药物丝裂霉素C和多柔比星对HepG2细胞的效率也显着增加暴露于磁场，虽然与最大的增强药物的疗效实现磁场之间的药物不同的时间。这些结果表明，60 Hz，50 mT的磁场增强抗癌药物对人癌HepG2细胞的作用。
Our previous data using bacterial cellsEscherichia colihave showed that 60 Hz, 50 mT magnetic fields enhance the efficacy of antibiotics. By using magnetic fields in clinical cancer chemotherapy, it is presumed to be able to enhance an anticancer drug efficacy on the target region, reduce dosage, and suppress side effects. However, since the prokaryotic bacterial cells, i.e.,E.coli, differ from human cells, which are eukaryotes and multicellular organisms in many ways, it was not clear whether the magnetic fields affect an efficacy of anticancer drug against human cancer cells. In this study, we designed and produced magnetic field exposure device for human cancer cells in culture and investigated whether 60 Hz, 50 mT magnetic fields affect the efficacy of anticancer drugs against human hepatoma HepG2 cells. The results of experiments with an anticancer drug cisplatin indicated that the quantity of viable HepG2 cells become decreased significantly by the combination of cisplatin and magnetic fields, as compared to that by cisplatin alone. This suggested that 60 Hz, 50 mT magnetic fields increase the cytocidal activity of cisplatin to human hepatoma cells. The efficiency of the anticancer drugs mitomycin C and doxorubicin against HepG2 cells was also increased significantly by exposure to magnetic fields, although the time associated with the greatest enhancement of the drug's efficacy achieved by magnetic fields differed among drugs. These results suggest that 60 Hz, 50 mT magnetic fields strengthen the effect of anticancer drugs on human cancer HepG2 cells.