Electrospinning Multilayered Scaffolds Loaded with Melatonin and Fe3O4 Magnetic Nanoparticles for Peripheral Nerve Regeneration
Electrospinning Multilayered Scaffolds Loaded with Melatonin and Fe3O4 Magnetic Nanoparticles for Peripheral Nerve Regeneration
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DOI：
10.1002/adfm.202004537
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发表时间：
2020-07-23
影响因子：
19
通讯作者：
Yuan, Wei-En
中科院分区：
文献类型：
--
作者：
Chen, Xuan;Ge, Xuemei;Yuan, Wei-En
周围神经损伤是临床上常见的问题，其发病率高，治疗效果不理想，给患者带来了沉重的负担。神经引导导管是一种很有前途的周围神经修复支架，应用生物活性物质可使神经功能恢复良好。褪黑激素（MLT）和Fe（3）O（4）磁性纳米颗粒（Fe 3 O 4-MNP）被证明可以抑制氧化应激，炎症，并诱导神经再生。在本文中，负载MLT和Fe 3 O 4-MNP的多层复合NGC被设计用于顺序和可持续的药物释放，为神经再生创造适当的微环境。该复合支架在体外表现出足够的机械强度和生物相容性，在体内可明显促进再生坐骨神经的形态、功能和电生理恢复。本工作证明，多层导管由于制作简单，疗效理想，在神经缺损的长期治疗中具有很大的前景。
Peripheral nerve injury is a common clinical problem bringing heavy burden to patients, due to its high incidence and unsatisfactory treatment. Nerve guidance conduit (NGC) is a promising scaffold for peripheral nerve repair, and bioactive agents are applied for great functional recovery. Melatonin (MLT) and Fe(3)O(4)magnetic nanoparticles (Fe3O4-MNPs) are proven to inhibit oxidative stress, inflammation, and induce nerve regeneration. Herein, a multilayered composite NGC loaded with MLT and Fe3O4-MNPs is designed for sequential and sustainable drug release, creating an appropriate microenvironment for nerve regeneration. The composite scaffold shows sufficient mechanical strength and biocompatibility in vitro, and evidently promotes morphological, functional, and electrophysiological recovery of regenerated sciatic nerves in vivo. This work proves that the multilayered conduits show great prospect in the long-term nerve defects treatment due to easy manufacture and desired efficacy.