KLUYVERA, A NEW (REDEFINED) GENUS IN THE FAMILY ENTEROBACTERIACEAE - IDENTIFICATION OF KLUYVERA-ASCORBATA SP-NOV AND KLUYVERA-CRYOCRESCENS SP-NOV IN CLINICAL SPECIMENS
KLUYVERA, A NEW (REDEFINED) GENUS IN THE FAMILY ENTEROBACTERIACEAE - IDENTIFICATION OF KLUYVERA-ASCORBATA SP-NOV AND KLUYVERA-CRYOCRESCENS SP-NOV IN CLINICAL SPECIMENS
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DOI：
10.1128/jcm.13.5.919-933.1981
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发表时间：
1981-01-01
影响因子：
9.4
通讯作者：
BRENNER, DJ
中科院分区：
文献类型：
--
作者：
FARMER, JJ;FANNING, GR;BRENNER, DJ
Kluyvera被提议为以前称为肠道菌群8（同义词= API 1组）的微生物组的新属。克鲁维拉菌株具有肠杆菌科大多数成员的特性：革兰氏阴性杆菌，可动，有周毛鞭毛，过氧化氢酶阳性，氧化酶阴性;它们在麦康凯琼脂上生长，发酵D-葡萄糖，产生酸和气体，对许多抗生素敏感。菌株通常为吲哚阳性、甲基红阳性、Voges-Proskauer阴性、柠檬酸盐阳性、H2S（三糖铁）阴性、尿素阴性、苯丙氨酸脱氨酶阴性、赖氨酸脱羧酶阳性、精氨酸二氢水解酶阴性和鸟氨酸脱羧酶阳性。Kluyvera菌株发酵许多用于鉴定的糖和多羟基醇。通过脱氧核糖核酸-脱氧核糖核酸杂交，将克鲁维拉菌分为3个类群。Kluyvera ascorbata被认为是该属的模式种。大多数菌株K.抗坏血酸已经从临床样品中分离出来。K. cryocrescens被认为是第二个物种。它偶尔从临床标本中分离出来，但更常见于从环境中分离出来。Kluyvera种组3是异质的，但通过脱氧核糖核酸杂交与两个命名的种不同。这一组是罕见的，所以没有物种名称将在这个时候提出。K.抗坏血酸可以与K.通过其阳性抗坏血酸测试，不能在冰箱中在5摄氏度下生长，以及羧苄青霉素和头孢噻吩圆盘周围较小的抑制区，可以确定cryocrescens的生长。通常用于鉴定的试验不能清楚区分这两个物种。Kluyvera种可能是罕见的机会致病菌。最常见的来源是痰，它们可能没有临床意义。五个菌株来自血培养。需要更多关于克鲁维拉属的发病率和临床意义的信息。
Kluyvera is proposed as a new genus for the group of organisms formerly known as Enteric Group 8 (synonym = API group 1). Strains of Kluyvera share the properties of most members of the family Enterobacteriaceae: they are gram-negative rods, motile with peritrichous flagella, catalase positive, and oxidase negative; they grow on MacConkey agar, ferment D-glucose with the production of acid and gas, and are susceptible to many antibiotics. Strains are usually indole positive, methyl red positive, Voges-Proskauer negative, citrate positive, H2S (triple sugar iron) negative, urea negative, phenylalanine deaminase negative, lysine decarboxylase positive, arginine dihydrolase negative, and ornithine decarboxylase positive. Kluyvera strains ferment many of the sugars and polyhydroxyl alcohols used in identification. By deoxyribonucleic acid-deoxyribonucleic acid hybridization, strains of Kluyvera were divided into three groups. Kluyvera ascorbata is proposed as the type species for the genus. Most strains of K. ascorbata have been isolated from clinical specimens. K. cryocrescens is proposed as the second species. It was occasionally isolated from clinical specimens, but it was isolated more commonly from the environment. Kluyvera species group 3 was heterogeneous, but was distinct from the two named species by deoxyribonucleic acid hybridization. This group was rare, so no species name will be proposed at this time. K. ascorbata can be differentiated from K. cryocrescens by its positive ascorbate test, inability to grow at 5 degrees C in a refrigerator, and smaller zones of inhibition around carbenicillin and cephalothin disks. The test normally used for identification does not clearly differentiate these two species. Kluyvera species are probably infrequent opportunistic pathogens. The most common source is sputum, where they are probably not clinically significant. Five strains have been from blood cultures. More information is needed about the incidence and clinical significance of the genus Kluyvera.