Correction of fluorescence detected circular dichroic photoselection artifacts using light collecting ellipsoidal mirrors
Correction of fluorescence detected circular dichroic photoselection artifacts using light collecting ellipsoidal mirrors
复制标题
使用聚光椭球镜校正荧光检测到的圆二向色光选择伪影
DOI：
10.1063/1.1139463
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发表时间：
1987
影响因子：
1.6
通讯作者：
M. Maestre
中科院分区：
文献类型：
--
作者：
S. Bicknese;M. Maestre
椭圆镜用于在荧光检测圆二色性（FDCD）测量中收集来自荧光团的发射光，并将该光以与激发光束成90°的角度传递到光电倍增管（PMT）。这些反射镜收集以样品池为中心的对称71%的发射球上的光，从而消除了由光选择的各向异性引起的检测到的发射中的大部分不均匀性。此外，即使有反射率和路径长度损失，到达PMT的荧光发射量也增加了约18倍。通过比较L-色氨酸在甘油中的光谱与其在水中的光谱，证明了UV FDCD光谱中光选择伪影的消除。测量值的有效性通过用d10三氟磺酸（d10 CSA）标准品校准进行验证。
Ellipsoidal mirrors are used to collect the emitted light from fluorophores in fluorescence detected circular dichroic (FDCD) measurements and to deliver this light to a photomultiplier tube (PMT) at 90° to the excitation beam. These mirrors collect light over a symmetrical 71% of the emission sphere centered on the sample cell, and thereby eliminate most of the nonuniformity in detected emission resulting from the anisotropy of photoselection. Furthermore, even with reflectance and path length losses, the amount of fluorescent emission reaching the PMT is increased by a factor of approximately 18. Elimination of photoselection artifacts in UV FDCD spectra is demonstrated by comparing spectra of L‐tryptophan in glycerol with its spectra in water. Validity of the values measured is verified through calibration with a d10 camphorsulfonic acid (d10 CSA) standard.