Bioconversion of plant biomass hydrolysate into bioplastic (polyhydroxyalkanoates) using Ralstonia eutropha 5119
Bioconversion of plant biomass hydrolysate into bioplastic (polyhydroxyalkanoates) using Ralstonia eutropha 5119
复制标题
DOI：
10.1016/j.biortech.2018.09.122
复制
发表时间：
2019-01-01
影响因子：
11.4
通讯作者：
Yang, Yung-Hun
中科院分区：
文献类型：
--
作者：
Bhatia, Shashi Kant;Gurav, Ranjit;Yang, Yung-Hun
木质纤维素生物质的预处理导致副产物（糠醛、羟甲基糠醛[HMF]、香草醛、乙酸酯等）的形成，其影响微生物生长和生产力。糠醛（0.02%）、HMF（0.04%）和乙酸盐（0.6%）对Ralstonia eutropha 5119的生长和聚羟基烷酸酯（PHA）的产生表现出积极的影响，而香草醛表现出消极的影响。响应优化和变量葡萄糖，氯化铵，糠醛，HMF，和乙酸之间的相互作用的研究，使用响应面法导致最大PHA生产（2.1 g/L）的最佳变量值分别为15.3 g/L，0.43 g/L，0.04 g/L，0.05 g/L，和2.34 g/L。以大麦、芒属植物和松树的木质纤维素生物质水解物为碳源，研究了不同木质纤维素生物质水解物对红曲霉生长的影响。eutropha 5119的PHA产量分别为1.8、2.0和1.7g/L。MBH为最佳碳源，生物量（Y-x/s，0.31g/g）和PHA（Y-p/s，0.14g/g）产量较高。
Pretreatment of lignocellulosic biomass results in the formation of byproducts (furfural, hydroxymethylfurfural [HMF], vanillin, acetate etc.), which affect microbial growth and productivity. Furfural (0.02%), HMF (0.04%), and acetate (0.6%) showed positive effects on Ralstonia eutropha 5119 growth and polyhydroxyalkanoate (PHA) production, while vanillin exhibited negative effects. Response optimization and interaction studies between the variables glucose, ammonium chloride, furfural, HMF, and acetate using the response surface methodology resulted in maximum PHA production (2.1 g/L) at optimal variable values of 15.3 g/L, 0.43 g/L, 0.04 g/L, 0.05 g/L, and 2.34 g/L, respectively. Different lignocellulosic biomass hydrolysates (LBHs), including barley biomass hydrolysate (BBH), Miscanthus biomass hydrolysate (MBH), and pine biomass hydrolysate (PBH), were evaluated as potential carbon sources for R. eutropha 5119 and resulted in 1.8, 2.0, and 1.7 g/L PHA production, respectively. MBH proved the best carbon source, resulted in higher biomass (Y-x/s, 0.31 g/g) and PHA (Y-p/s, 0.14 g/g) yield.