Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.
Cloning and characterization of a photolyase gene from the cyanobacterium Anacystis nidulans.
复制标题
蓝藻 Anacystis nidulans 光裂合酶基因的克隆和表征。
作者：
A. Yasui;M. Takao;A. Oikawa;A. Kiener;C. Walsh;A. Eker
通过与衍生自 Anacystis 光裂合酶 N 端氨基酸序列的合成寡核苷酸探针杂交，从 Anacystis nidulans 基因组 DNA 文库中分离出 2 kb 片段。该片段包含一个 1452 bp 长的开放阅读框，编码 484 个氨基酸的多肽（Mr 54475）。针对纯化的 Anacystis 光裂合酶产生的抗体与含有大肠杆菌 lacZ 和该基因之间融合基因的细胞提取物发生反应。尽管发色团结构存在差异，但推导的 Anacystis 和大肠杆菌光裂合酶的氨基酸序列之间存在 40.7% 的相似性。
A 2 kb fragment was isolated from an Anacystis nidulans genomic DNA library by hybridization with synthetic oligonucleotide probes derived from the N-terminal amino acid sequence of Anacystis photolyase. This fragment contains a 1452 bp-long open reading frame encoding a polypeptide of 484 amino acids (Mr 54475). Antibodies raised against purified Anacystis photolyase reacted with extracts of cells harboring fused genes between lacZ of Escherichia coli and this gene. A 40.7% similarity was found between the deduced amino acid sequences of Anacystis and E. coli photolyases, notwithstanding the difference in chromophore structure.