Contribution of extracellular substances to cell protective abilities against UV radiation and differentiation of germination, vegetative cells and hormogonia, during their life cycle in <i>Nostoc</i> sp. HK-01
Contribution of extracellular substances to cell protective abilities against UV radiation and differentiation of germination, vegetative cells and hormogonia, during their life cycle in <i>Nostoc</i> sp. HK-01
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发菜生命周期中，细胞外物质对细胞抵御紫外线辐射的保护能力以及发芽、营养细胞和激素的分化的贡献。
DOI：
10.11450/seitaikogaku.35.13
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发表时间：
2023
期刊：
影响因子：
0.3
通讯作者：
Kaori Tomita-Yokotani
中科院分区：
文献类型：
--
作者：
Midori Ong;Miku Tokita;Hiroshi Katoh;Tomoko Abe;Sayaka Takahashi;Hajime Mita;Kazumichi Nakagawa;Toshisada Suzuki;Kaori Tomita-Yokotani
研究了陆生蓝藻（Nostoc sp. HK-01）细胞外物质对细胞抗紫外线（UV）的保护作用。这种先进的方法被成功地应用于防紫外线生物测定。将带ESs和不带ESs的细胞暴露于紫外线下，结果表明，带ESs的细胞存活率高于不带ESs的细胞。Nostoc sp. HK-01的ES未表现出特异性反射；然而，对其在紫外波段，特别是UV- c波段的高吸收率进行了研究。结果表明，ES中存在具有防UV-C性能的物质。此外，我们还证实了ES中与细胞分化有关的其他功能，即肌细胞萌发、向营养细胞分化和向同源细胞分化。这一现象只在有限浓度的ES中出现。在发芽试验中，ESs中元素的贡献有很大的影响；但某些有机化合物具有一定的抑制和促进作用。在芽孢萌发的早期，首次检测并证实了ESs在芽孢属中分化的发生。我们的研究结果表明，Nostoc sp. HK-01细胞在恶劣环境条件下可能具有独特的细胞水化和干燥能力。
The functions of extracellular substances (ESs) that contribute to the protection of cells against ultraviolet (UV) radiation were investigated in the terrestrial cyanobacterium, Nostoc sp. HK-01. The original advanced method was used successfully and was found useful for UV-protective bioassay. Cells with and without ESs were exposed to UV radiation, and results showed the survival rate of the cells with ESs was higher than that of the cells without ESs. The ES of Nostoc sp. HK-01 did not show the specific reflection; however, the high absorption in the UV range, especially UV-C range, was investigated. The results indicated the presence of substances with UV-C protection properties in the ES. Additionally, we confirmed other functions related to cell differentiation, namely, akinete germination, differentiation to vegetative cells, and differentiation to hormogonia, in ES. This phenomenon showed in a limited concentration of ES. In the germination test, contribution of elements in ESs had great effect; however, some inhibiting and promoting effects were confirmed as some organic compound. The occurrence of differentiation in hormogonia by ESs was first examined and confirmed during the early stages of akinete germination. Our results demonstrated that Nostoc sp. HK-01 cells may have unique abilities during cell hydration and drying under harsh environmental conditions.