Passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys.
Passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys.
复制标题
恒河猴的免疫球蛋白从血浆到口腔的传递。
作者：
S. Challacombe;M. Russell;J. Hawkes;L. Bergmeier;T. Lehner
在恒河猴中研究了免疫球蛋白从血浆到口腔的通道。从合并的恒河猴血清中纯化免疫球蛋白G、A和M，用125I放射性标记并静脉注射到12只猴子中。在24小时内连续采集口腔液样品，并测定放射性。在注射IgG和伊加的猴中，0.5 h后，以及在注射IgM的猴中，2 h后，可在龈沟液冲洗液中检测到放射性。每种免疫球蛋白在4小时后均达到最大水平。30 min后，所有动物的腮腺和混合唾液中均可检测到放射性，4 h后达到最高水平。蔗糖密度梯度的超离心显示，给予IgG和伊加的动物中，龈沟液冲洗液中的大部分放射性位于7S区，给予IgM的动物中，位于19S区。腮腺唾液中的放射性并不代表完整的免疫球蛋白分子，因为所有活性都存在于给予IgG、伊加或IgM的动物的低分子量区域中。在混合唾液中，在免疫球蛋白区发现少量放射性。结果表明，完整的IgG，伊加和IgM分子可以通过龈沟液从血浆到口腔，并可能有助于口腔防御机制，特别是在龈沟域。恒河猴乳磨牙近端间隙中的龈沟液体积估计约为0.3微升。
The passage of immunoglobulin from plasma to the oral cavity was studied in rhesus monkeys. Immunoglobulins G, A and M were purified from pooled rhesus monkey serum, radiolabelled with 125I and injected intravenously into twelve monkeys. Sequential samples of oral fluids were taken over a 24 h period and were assayed for radioactivity. Radioactivity could be detected in crevicular fluid washings after 0.5 h in monkeys injected with IgG and IgA, and after 2 h in monkeys given IgM. Maximal levels were found after 4 h with each immunoglobulin. Radioactivity in parotid and mixed saliva could be detected in all animals after 30 min, reaching a maximal level after 4 h. Ultracentrifugation on sucrose density gradients revealed that most of the radioactivity in crevicular fluid washings was in the 7S zone in the animals given IgG and IgA, and in a 19S zone in animals given IgM. The radioactivity in partoid saliva did not represent intact immunoglobulin molecules, since all the activity was present in zones of low molecular weight in animals given IgG, IgA or IgM. In mixed saliva a small amount of radioactivity was found in the immunoglobulin zones. The results suggest that intact molecules of IgG, IgA and IgM can pass from plasma to the oral cavity via crevicular fluid, and could contribute to oral defence mechanisms particularly in the crevicular domain. The volume of crevicular fluid in the approximal space of deciduous molars of rhesus monkeys was estimated to be approximately 0.3 microliter.