Enrichment of hematopoietic precursor cells and cloning of multipotential B-lymphocyte precursors.
Enrichment of hematopoietic precursor cells and cloning of multipotential B-lymphocyte precursors.
复制标题
造血前体细胞的富集和多能 B 淋巴细胞前体细胞的克隆。
DOI：
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发表时间：
1985
影响因子：
11.1
通讯作者：
B. Fagg
中科院分区：
文献类型：
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作者：
J. Mckearn;J. McCubrey;B. Fagg
一个简单的一步分离技术显着富集小鼠胎肝细胞，响应白细胞介素3（IL-3），多系造血生长因子。用单克隆抗体AA 4分离的胎肝细胞亚群含有50- 100倍高频率的多能（CFU-混合物）或限制性（CFU-G/M，BFU-E）红细胞/髓细胞前体以及分化为成熟B淋巴细胞的前体[CFU-混合物=红细胞和髓细胞集落形成单位; CFU-G/M = CFU-粒细胞/巨噬细胞; BFU-E =爆发形成单位-红细胞]。当含有IL-3的上清液存在时，B淋巴细胞前体可以在单细胞培养物中克隆。这些克隆的生长由纯化的IL-3支持，但不由纯化的IL-2支持。在IL-3的存在下，稳定生长已维持超过6个月。这些克隆在它们的细胞表面上表达少量的I类主要组织相容性复合物抗原和大量的AA 4、GF 1和白细胞共同糖蛋白200抗原。即使在亚克隆后，其Ig编码基因[连接区重链和轻链（κ，λ）基因]也缺乏可检测的重排，但仍保持其分化为成熟B淋巴细胞的能力，这些淋巴细胞具有多种IG特异性。
A simple one-step isolation technique significantly enriched mouse fetal liver cells that respond to interleukin 3 (IL-3), a multilineage hematopoietic growth factor. The fetal liver cell subpopulation isolated with monoclonal antibody AA4 contained 50- to 100-fold higher frequencies of multipotential (CFU-mix) or restricted (CFU-G/M, BFU-E) erythroid/myeloid precursors as well as precursors that differentiate to become mature B lymphocytes [CFU-mix = erythroid and myeloid colony-forming unit(s); CFU-G/M = CFU-granulocyte/macrophage; BFU-E = burst-forming unit-erythroid]. The B-lymphocyte precursors could be cloned in single-cell cultures when IL-3-containing supernatants were present. Growth of these clones was supported by purified IL-3 but not by purified IL-2. Stable growth has been maintained for greater than 6 mo in the presence of IL-3. Such clones express on their cell surface low amounts of class I major histocompatibility complex antigens and high amounts of AA4, GF1, and leukocyte common glycoprotein 200 antigens. They lack detectable rearrangements of their Ig-encoding genes [joining region heavy and light (kappa, lambda) chain genes], even after subcloning, but maintain their capacity to differentiate to mature B lymphocytes committed to multiple Ig specificities.