Catalytic activities of the human T-cell leukemia virus type II integrase.
Catalytic activities of the human T-cell leukemia virus type II integrase.
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人类 T 细胞白血病病毒 II 型整合酶的催化活性。
DOI：
10.1006/viro.1996.0224
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发表时间：
1996
期刊：
影响因子：
3.7
通讯作者：
Jonsson,CB
中科院分区：
文献类型：
--
作者：
Balakrishnan,M;Zastrow,D;Jonsson,CB
尽管HTLV-II在新世界人群和静脉注射毒品使用者中广泛存在，但还没有报道过thepolgenes的酶活性。为了确定HTLV-IIG 12整合酶（IN）的活性，分离编码区，并使用镍亲和层析将编码的蛋白质纯化至大于90%的均一性。HTLV-IIG 12 IN被证明对HTLV-IIG 12和HIV-1整合和崩解底物具有活性。观察到3′-加工、链转移和崩解反应对酶浓度的要求存在明显差异。整合反应的催化发生在Mn 2+或Mg 2+的存在下，虽然链转移活性优选Mn 2+。相比之下，HTLV-IIG 12 IN催化崩解反应时蛋白质几乎少10倍，对Mn 2+或Mg 2+没有选择性，并且耐受比整合更高的NaCl浓度。HTLV-IIG 12 IN不能催化“剪接”反应，这表明这可能不是所有逆转录病毒整合酶普遍存在的活性。
Despite the widespread nature of HTLV-II in New World populations and intravenous drug users, the enzymatic activities of thepolgenes have not been reported. To ascertain the activity of the HTLV-IIG12integrase (IN), the coding region was isolated and the encoded protein was purified, using nickel-affinity chromatography, to greater than 90% homogeneity. HTLV-IIG12IN proved active on HTLV-IIG12and HIV-1 integration and disintegration substrates. Distinct differences in requirements for enzyme concentration for 3′-processing, strand-transfer, and disintegration reactions were observed. Catalysis of integration reactions occurred in the presence of either Mn2+or Mg2+, although strand-transfer activity preferred Mn2+. In comparison, HTLV-IIG12IN catalyzed disintegration reactions with almost 10-fold less protein, was not selective for Mn2+or Mg2+, and tolerated higher NaCl concentrations than integration. HTLV-IIG12IN was unable to catalyze the “splicing” reaction, which suggests that this may not be an activity ubiquitous to all retroviral integrases.