High-dimensional and large-scale phenotyping of yeast mutants
High-dimensional and large-scale phenotyping of yeast mutants
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DOI：
10.1073/pnas.0509436102
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发表时间：
2005-12-27
影响因子：
11.1
通讯作者：
Morishita, S
中科院分区：
文献类型：
--
作者：
Ohya, Y;Sese, J;Morishita, S
在单细胞和多细胞生物体中，将功能归于基因的最强大的技术之一是对突变特征的综合分析。本研究通过对酿酒酵母的形态表型进行研究，对其突变性状进行了系统的、定量的分析。对三重染色细胞的荧光显微图像的分析使将形态变异作为数量性状处理成为可能。近一半对生长不重要的酵母基因的缺失会影响这些形态特征。相似的形态表型是由功能相关基因的缺失引起的，这使得一个基因座能够在功能上分配到特定的细胞途径。对已定义的酵母突变菌株的高维表型分析提供了将基因功能归因于酵母基因组中所有基因的又一步。
One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.