The Extracellular Matrix Protein TGFBI promotes Myofibril Bundling and Muscle Fibre Growth in the Zebrafish Embryo
The Extracellular Matrix Protein TGFBI promotes Myofibril Bundling and Muscle Fibre Growth in the Zebrafish Embryo
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DOI：
10.1002/dvdy.21812
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发表时间：
2009-01-01
影响因子：
2.5
通讯作者：
Ingham, Philip W.
中科院分区：
文献类型：
--
作者：
Kim, H. Rosemary;Ingham, Philip W.
细胞外基质蛋白 Tgfbi 已被证明定位于小鼠的肌腱连接处，并推测与跨膜蛋白整合素 α 7 β 1 相互作用，后者与肌营养不良蛋白相关蛋白复合物平行，对于细胞外基质和肌纤维细胞骨架之间的连接至关重要。在这里，我们使用 GFP 标记形式的 Tgfbi 来分析其在斑马鱼胚胎发育中的骨骼肌中的分布，并使用反义吗啉寡核苷酸来研究内源蛋白的功能。我们发现，尽管标记的 Tgfbi 在肌隔膜处积累，但在 morphant 胚胎中，肌纤维与肌隔膜的附着仍然正常建立和维持；然而，纤维的生长明显减少，肌原纤维束受到破坏。发育动力学 238:56-65, 2009。(C) 2008 Wiley-Liss, Inc.
The extracellular matrix protein Tgfbi has been shown to localise to myotendinous junctions in mouse and postulated to interact with the transmembrane protein Integrin alpha 7 beta 1, which, in parallel with the Dystrophin-associated protein complex, is critical for linkage between the extracellular matrix and the cytoskeleton of muscle fibres. Here we use a GFP-tagged form of Tgfbi to analyse its distribution in the developing skeletal muscle of the zebrafish embryos and antisense morpholino oligonucleotides to investigate the function of the endogenous protein. We find that although tagged Tgfbi accumulates at the myosepta, the attachment of muscle fibres to the myosepta is established and maintained normally in morphant embryos; however, the fibres show a marked reduction in their growth and a disruption of their myofibril bundles. Developmental Dynamics 238:56-65, 2009. (C) 2008 Wiley-Liss, Inc.