Utilization and Toxicity of Exogenous Amino Acids in Euglena gracilis

Utilization and Toxicity of Exogenous Amino Acids in Euglena gracilis
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眼虫外源氨基酸的利用及毒性

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发表时间:
2008
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通讯作者:
Y. O S H I H I S A N A K A N O A N D S H O Z A B U R
Y. O S H I H I S A N A K A N O A N D S H O Z A B U R
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作者:
Y. O S H I H I S A N A K A N O A N D S H O Z A B U R

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以乙醇为碳源,研究了纤细裸藻Z株和链霉素漂白突变株SM-ZK对氨基酸的利用。在21种氨基酸中,谷氨酸是所有条件下两种菌株最有效的营养素。蛋氨酸、半胱氨酸、苏氨酸、亮氨酸和苯丙氨酸对E.纤细蟹以谷氨酸为碳源和氮源,但这些氨基酸既不抑制谷氨酸的转运,也不抑制呼吸。蛋氨酸、苏氨酸和苯丙氨酸的生长抑制作用分别被甘氨酸、缬氨酸和色氨酸特异性地解除。
Amino acid utilization by Euglena gracilis strains z and SM-ZK (a streptomycin-bleached mutant) was studied in the presence or absence of ethanol as a carbon source. Among 21 amino acids examined, glutamate was the most effective nutrient for both strains under all conditions. Methionine, cysteine, threonine, leucine and phenylalanine inhibited the growth of E. gracilis on glutamate as the carbon and nitrogen source, but neither glutamate transport nor respiration was inhibited by these amino acids. The growth inhibition by methionine, threonine and phenylalanine were specifically relieved by glycine, valine and tryptophan, respectively.