Hippocampal neurogenesis in adult Old World primates
Hippocampal neurogenesis in adult Old World primates
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DOI：
10.1073/pnas.96.9.5263
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发表时间：
1999-04-27
影响因子：
11.1
通讯作者：
Fuchs, E
中科院分区：
文献类型：
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作者：
Gould, E;Reeves, AJ;Fuchs, E
在啮齿动物中，成年期新海马神经元的产生已得到充分记录。最近的研究将这些发现扩展到其他哺乳动物物种，例如树鼩和狨猴。然而，海马神经发生尚未在成年旧大陆灵长类动物中得到证实。为了研究这种可能性，我们给11只不同年龄（5-23岁）的成年旧世界猴注射了胸苷类似物溴脱氧尿苷，并使用神经元和神经胶质标记物检查了标记细胞在不同存活时间的命运。在年轻成年和中年猴子中，我们发现大量细胞掺入了溴脱氧尿苷，并表现出未成熟和成熟神经元的形态和生化特征。位于齿状回的新细胞表达未成熟颗粒神经元的标记，分裂后开启64 kDa蛋白，以及成熟颗粒神经元的标记，包括神经元特异性烯醇化酶、神经元核和钙结合蛋白钙结合蛋白。较少的新细胞表达星形胶质细胞标记神经胶质纤维酸性蛋白。在最年长的猴子（23 岁）中也观察到了神经发生的证据，但似乎不太稳健。这些结果表明，旧世界猴的成年大脑产生了新的海马神经元，成年猕猴可能为研究成年神经发生的功能意义提供有用的灵长类动物模型。
The production of new hippocampal neurons in adulthood has been well documented in rodents. Recent studies have extended these findings to other mammalian species, such as tree shrews and marmoset monkeys. However, hippocampal neurogenesis has not been demonstrated in adult Old World primates. To investigate this possibility, we injected 11 adult Old World monkeys of different ages (5-23 years) with the thymidine analog bromodeoxyuridine and examined the fate of the labeled cells at different survival times by using neuronal and glial markers, In the young-adult and middle-aged monkeys, we found a substantial number of cells that incorporated bromodeoxyuridine and exhibited morphological and biochemical characteristics of immature and mature neurons. New cells located in the dentate gyrus expressed a marker of immature granule neurons, Turned On After Division 64 kDa protein, as well as markers of mature granule neurons including neuron specific enolase, neuronal nuclei, and the calcium-binding protein calbindin, Fewer new cells expressed the astroglial marker glial fibrillary acidic protein. Evidence of neurogenesis was observed in the oldest monkeys (23 years) as well, but it appeared to be less robust. These results indicate that the adult brains of Old World monkeys produce new hippocampal neurons, Adult macaque monkeys may provide a useful primate model for studying the functional significance of adult neurogenesis.