Kinetic characterization of a slow chemical exchange between two sites in N,N-dimethylacetylamide by CEST NMR spectroscopy
Kinetic characterization of a slow chemical exchange between two sites in N,N-dimethylacetylamide by CEST NMR spectroscopy
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DOI：
10.1016/j.cclet.2022.01.022
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发表时间：
2022-01
影响因子：
9.1
通讯作者：
Li-Xia Wang;Jikun Li;J. Xiang;Jie Cui;Yalin Tang
中科院分区：
文献类型：
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作者：
Li-Xia Wang;Jikun Li;J. Xiang;Jie Cui;Yalin Tang
Nuclear magnetic resonance (NMR) spectroscopy has provided many powerful tools for the study of dynamic processes. Among the reported methods, chemical exchange saturation transfer (CEST) is more suitable for systems with slow exchange rates, and there will be promising in the detection and dynamic mechanism of metastable substances. It has been widely used in magnetic resonance imaging (MRI), however whether it is applicable in the field of chemical kinetics needs more examples. Here we studied, as a proof of concept, the kinetics of the slow chemical exchange between the twoN-methyl protons ofN,N-dimethylacetylamide (DMA), exploiting QUantifying Exchange using Z-spectrum (QUEZS) and QUantifying Exchange using Saturation Time (QUEST) methods. It turned out that both of QUEZS and QUEST could give the corresponding exchange rates, showcasing the capability of this method to provide accurate kinetic data under a range of temperatures. Our results clearly demonstrated the reliability of CEST-based techniques as a tool for dynamic kinetics by NMR.
