Bacteriophytochromes: Phytochrome-like photoreceptors from nonphotosynthetic eubacteria
Bacteriophytochromes: Phytochrome-like photoreceptors from nonphotosynthetic eubacteria
复制标题
DOI：
10.1126/science.286.5449.2517
复制
发表时间：
1999-12-24
期刊：
影响因子：
56.9
通讯作者：
Vierstra, RD
中科院分区：
文献类型：
--
作者：
Davis, SJ;Vener, AV;Vierstra, RD
光敏色素是绿色植物用来将其发育带入光环境的光感受器家族。这些色蛋白的分布已经扩大到光自养生物以外，在非光合真细菌Deinococcus radiodurans和铜绿假单胞菌中发现了光敏色素样蛋白。与植物光敏色素一样，D.抗辐射受体共价结合线性四吡咯自催化产生光致变色全蛋白。然而，连接位点是不同的，使用组氨酸以潜在地形成席夫碱连接。序列同源性和突变分析表明，D.耐辐射细菌光敏色素作为光调节组氨酸激酶发挥作用，有助于保护细菌免受可见光的影响。
Phytochromes are a family of photoreceptors used by green plants to entrain their development to the light environment. The distribution of these chromoproteins has been expanded beyond photoautotrophs with the discovery of phytochrome-like proteins in the nonphotosynthetic eubacteria Deinococcus radiodurans and Pseudomonas aeruginosa. Like plant phytochromes, the D. radiodurans receptor covalently binds Linear tetrapyrroles autocatalytically to generate a photochromic holoprotein. However, the attachment site is distinct, using a histidine to potentially form a Schiff base Linkage. Sequence homology and mutational analysis suggest that D. radiodurans bacteriophytochrome functions as a Light-regulated histidine kinase, which helps protect the bacterium from visible Light.