Laboratory Diagnosis of Noroviruses: Present and Future
Laboratory Diagnosis of Noroviruses: Present and Future
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DOI：
10.1016/j.cll.2015.02.008
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发表时间：
2015-06-01
影响因子：
1.7
通讯作者：
Lee, Bonita E.
中科院分区：
文献类型：
--
作者：
Pang, Xiaoli;Lee, Bonita E.
诺沃克病毒是全球胃肠炎暴发的重要原因，也是许多地区散发性胃肠炎的最常见原因。快速准确地识别致病病原体对于暴发调查、疾病监测和管理至关重要。由于诺沃克病毒不是可培养的，而且基因组高度多样化和可变，因此很难开发诊断分析方法。检测方法已经从电子显微镜发展到传统的终点逆转录聚合酶链式反应(RT-PCR)、免疫分析、实时RT-PCR、其他分子技术和基于纳米技术的阵列分析。本文综述了各种检测方法的现状和特点。
Norovirus is an important cause of gastroenteritis outbreaks globally and the most prevalent cause of sporadic gastroenteritis in many regions. Rapid and accurate identification of causative viral agents is critical for outbreak investigation, disease surveillance, and management. Because norovirus is not cultivable and has a highly diversified and variable genome, it is difficult to develop diagnostic assays. Detection methods have evolved from electron microscopy to conventional end-point reverse transcription polymerase chain reaction (RT-PCR), immunoassay, real-time RT-PCR, other molecular technologies, and nanotechnology array-based assays. The status and features of various testing methods are summarized in this review.