Search for methylation-sensitive amplification polymorphisms associated with the "mantled" variant phenotype in oil palm (Elaeis guineensis Jacq.)
Search for methylation-sensitive amplification polymorphisms associated with the "mantled" variant phenotype in oil palm (Elaeis guineensis Jacq.)
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作者：
Jaligot, E;Beulé, T;Rival, A
利用甲基化敏感扩增多态性（MSAP）技术鉴定了油棕体细胞胚源性体细胞无性系变异相关的甲基化多态性。变异表型在雄花和雌花中都表现出不稳定的雄性器官雌性化。利用MSAP，比较了四个基因典型不同的母棕榈树体细胞胚胎发生获得的克隆群体中三个正常再生体和三个覆盖再生体CCGG位点的甲基化状态。总体而言，使用了64个选择性引物组合，扩增出了23个标记，显示出两种表型之间的差异甲基化模式。我们的研究结果表明，CCGG位点很少受到先前与覆盖表型相关的全球DNA甲基化显著减少的影响。在本研究中分离的23个标记中的每一个都可以区分两种表型，只有当它们来自相同的遗传来源时。这一结果目前阻碍了直接使用MSAP标记进行变异的早期检测，尽管从这些多态标记的进一步表征中将获得关于假定目标序列的有价值信息。
The methylation-sensitive amplification polymorphism (MSAP) technique has been employed on somatic embryo-derived oil palms (Elaeis guineensis Jacq.) to identify methylation polymorphisms correlated with the "mantled" somaclonal variation. The variant phenotype displays an unstable feminization of male organs in both male and female flowers. Using MSAP, the methylation status of CCGG sites was compared in three normal versus three mantled regenerants sampled in clonal populations obtained through somatic embryogenesis from four genotypically distinct mother palms. Overall, 64 selective primer combinations were used and they have amplified 23 markers exhibiting a differential methylation pattern between the two phenotypes. Our results indicate that CCGG sites are poorly affected by the considerable decrease in global DNA methylation that has been previously associated with the mantled phenotype. Each of the 23 markers isolated in the present study could discriminate between the two phenotypes only when they were from the same genetic origin. This result hampers at the moment the direct use of MSAP markers for the early detection of variants, even though valuable information on putative target sequences will be obtained from a further characterization of these polymorphic markers.