Tissue distributions of CYP2D1, 2D2, 2D3 and 2D4 mRNA in rats detected by RT-PCR
Tissue distributions of CYP2D1, 2D2, 2D3 and 2D4 mRNA in rats detected by RT-PCR
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DOI：
10.1016/s0304-4165(97)00157-8
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发表时间：
1998-05-08
影响因子：
3
通讯作者：
Funae, Y
中科院分区：
文献类型：
--
作者：
Hiroi, T;Imaoka, S;Funae, Y
研究了四种亚型（CYP2D1/5、2D2、2D3 和 2D4/18）在大鼠 CYP2D 亚家族中的组织分布。从Sprague-Dawley雄性和雌性大鼠身上摘取12种组织（肝、肾、脑、肺、心脏、脾、肾上腺、小肠粘膜、膀胱、睾丸、卵巢和去囊肿）。 RT-PCR检测这些组织中CYP2D mRNA的表达。设计特异性引物来分别识别四种亚型。在肝脏、肾脏和小肠粘膜中，所有四种 CYP2D 亚型的 mRNA 表达均以高强度 PCR 产物的形式检测到。尽管 PCR 产物的强度因组织而异，但 CYP2D1/5 的 mRNA 在本研究中除脑外的所有组织中均表达。 CYP2D2和CYP2D3的mRNA主要在肝、肾和小肠粘膜中表达，这些区域暴露于药物、食品成分和环境污染物等外源物质。 CYP2D4/18 mRNA在肝、肾、小肠粘膜和脑中表达。在大脑中，仅表达 CYP2D4/18 的 mRNA。 CYP2D4/18 mRNA也在卵巢、睾丸和去囊肿中表达。组织分布有助于阐明 CYP2D 亚型之间生理和药理功能的差异。 (C) 1998 Elsevier Science B.V.
The tissue distributions of four isoforms (CYP2D1/5, 2D2, 2D3 and 2D4/18) in rat CYP2D subfamily were investigated. Twelve kinds of tissue (liver, kidney, brain, lung, heart, spleen, adrenal gland, small intestine mucosa, bladder, testis, ovary and gonecystis) were removed from Sprague-Dawley male and female rats. The expression of CYP2D mRNA in these tissues was detected by RT-PCR. Specific primers were designed to recognize the four isoforms individually. In liver, kidney and small intestine mucosa, the mRNA expression of all four CYP2D isoforms was detected as high-intensity PCR products. mRNA of CYP2D1/5 was expressed in all tissues used in this study except the brain, although the intensity of PCR products varied among tissues. mRNAs of CYP2D2 and CYP2D3 were mainly expressed in liver, kidney and small intestine mucosa, which were exposed to xenobiotics such as drugs, food components and environmental contaminations. mRNA of CYP2D4/18 was expressed in liver, kidney, small intestine mucosa and brain. In brain, only mRNA of CYP2D4/18 was expressed. CYP2D4/18 mRNA was also expressed in ovary, testis and gonecystis. The tissue distributions help to clarify the differences in physiological and pharmacological functions between CYP2D isoforms. (C) 1998 Elsevier Science B.V.